Anti brachyury

Manufactured by Santa Cruz Biotechnology

Anti-brachyury is a laboratory reagent manufactured by Santa Cruz Biotechnology. It is an antibody that targets the brachyury protein, which plays a role in the development of certain cell lineages. The primary function of Anti-brachyury is to facilitate the detection and study of brachyury in biological samples.

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Lab products found in correlation

4 protocols using anti brachyury

Comprehensive Protein Expression Analysis

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The following antibodies were used: Anti-EGFR (Santa Cruz sc-03 rabbit polyclonal); anti-PDGFRβ (Cell Signaling 4564 rabbit monoclonal); anti-MET (Santa Cruz sc10 rabbit polyclonal); anti-P-AKT (Cell Signaling 4060 rabbit monoclonal); anti- AKT (Cell Signaling – cs 9272); anti-P-STAT3 (Cell Signaling 9131 rabbit polyclonal); anti-STAT3 (Cell Signaling – cs 9139); anti-brachyury (Santa Cruz sc-20109 rabbit polyclonal), anti- GAPDH (Santa Cruz 25778 rabbit polyclonal).
HRP-conjugated secondary antibodies were used 1:10000 (Immunopure goat anti-mouse and Immunopure Goat Anti-rabbit from Thermo-Scientific). Detection was performed using SuperSignal west Pico Chemiluminescent substrate from Thermo Scientific

Bosotti R., Magnaghi P., Di Bella S., Cozzi L., Cusi C., Bozzi F., Beltrami N., Carapezza G., Ballinari D., Amboldi N., Lupi R., Somaschini A., Raddrizzani L., Salom B., Galvani A., Stacchiotti S., Tamborini E, & Isacchi A. (2017). Establishment and genomic characterization of the new chordoma cell line Chor-IN-1. Scientific Reports, 7, 9226.

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Pluripotency Protein Analysis Protocol

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Smc3 (Abcam, Ab9263), Sall4 (Abcam, Ab29112), Wapal (Abcam, Ab70741), Scc1/Rad21 (Abcam, Ab992), Nanog (Millipore, AB5731), GAPDH-HRP (Santa Cruz, sc-25778), total H3 (Active Motif, 61278), H3K27me3 (Millipore, 07-449), H3K4me3 (Active Motif, 39916), anti-Brachyury (Santa Cruz, sc-17743), anti-v5 (Invitrogen, 46-0708), Streptavidin–HRP (Invitrogen, SA1007), anti-Suz12 (Santa Cruz, sc-46264), anti-Ezh2 (Millipore, 17-662), anti-Ring1b (Western Blot-Active Motif, 39664; ChIP-seq Abcam, Ab101273), anti-H2Aub1 (Cell Signaling, 8240).

Stelloh C., Reimer M.H., Pulakanti K., Blinka S., Peterson J., Pinello L., Jia S., Roumiantsev S., Hessner M.J., Milanovich S., Yuan G.C, & Rao S. (2016). The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics & Chromatin, 9, 14.

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Lipid-based Nanoparticle Formulation

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N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (DOTAP) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-5000] were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol was obtained from Sigma (St Louis, MO, USA). Protamine sulfate-USP was from Eli Lilly (St Louis. MO, USA). Anti-brachyury and anti-HRP conjugated secondary antibody against rabbit were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Luciferase assay kit, CellTiter 96^® AQueous One solution Assay and Caspasc-Glo 3/7 Assay were from Promega (Madison, WI, USA).

Hu Y., Singh R., Deng Z., Mintz A, & Hsu W. (2016). Liposome-Protamine-DNA Nanoparticle-Mediated Delivery of Short Hairpin RNA Targeting Brachyury Inhibits Chordoma Cell Growth. Journal of biomedical nanotechnology, 12(10), 1952-1961.

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Comprehensive Protein Analysis Workflow

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Western blots were performed by separating proteins on SDS-PAGE gels followed by protein transfer to PVDF membranes (Bio-Rad). The antibodies used were: anti-PDGFD (sc-137030, Santa Cruz), anti-SOX2 (AF-2018, R&D), anti-BRACHYURY (sc-166962, Santa Cruz), anti-ERKs (4696, Cell Signaling Technology), anti-Phospho ERKs (4370, Cell Signaling Technology), anti-PDGFR-β (3169, Cell Signaling Technology), anti-Phospho PDGFR-β (Tyr1021) (2227, Cell Signaling Technology), anti-PECAM1 (222783, Abcom), anti-Phospho STAT3 (9145, Cell Signaling Technology), anti-STAT3 (9139, Cell Signaling Technology), anti-KDR (9698, Cell Signaling Technology), anti-HSP90 (7411, Cell Signaling Technology), anti-TUBULIN (RM2007, Ray Antibody), anti-β-ACTIN (RM2001, Ray Antibody), anti-GAPDH (70-Mab5465-040, MultiSciences), anti-HISTONE 3 (GB13102-1, Servicebio), Goat anti-mouse IgG (GAM0072, MultiSciences), Goat anti-rabbit IgG (GAR0072, MultiSciences), Rabbit anti-goat IgG (RAG0072, MultiSciences), anti-PDGFB (PA1-27394, Invitrogen), anti-uPA (17968-1-AP, Proteintech) and anti-PLASMIN (66399-1-Ig, Proteintech). The bands were visualized using a GBOX-CHEMI-XX8 device (SYNGENE).

Lu W., Xu P., Deng B., Zhang J., Zhan Y., Lin X., Xu X., Xia Z., Yang X., Zeng X., Huang L., Xie B., Wang C., Wang S., Kuang H., Han X., Mora A., Cao Y., Jiang Q, & Li X. (2022). PDGFD switches on stem cell endothelial commitment. Angiogenesis, 25(4), 517-533.

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