Cathepsin b antibody

Quercetin (purity ≥ 95%) and desferrioxamine (DFO) were from Sigma (USA). Collagenase (type IV), silver-lactate, bafilomycin A1, acridine orange (AO), and tetramethylrhodamine methyl ester (TMRM) were purchased from Sigma-Aldrich (USA). Dulbecco's Modified Eagle's Medium (DMEM) was obtained from Gibco (USA). In situ cell death detection kit was provided by Roche (Switzerland). Ethanol and FeCl₃ were purchased from Zhenxing Chemical Factory (Shanghai, China). Dihydroethidium (DHE), 2′,7′-Dichlorodihydrofluorescein diacetate (H₂DCFDA), and LysoTracker Red were purchased from Beyotime Corporation (Jiangsu, China). Cathepsin B activity assay kit and apoptosis assay kit were from BioVision (USA) and KenGEN BioTECH (Nanjing, China), respectively. GSH/GSSH assay kit was purchased from the Nanjing Jiancheng Corp., China. Cleaved caspase-3 antibody and cytochrome C antibody were purchased from CST (USA). Cathepsin B antibody and rabbit ferritin antibody were from Santa Cruz (USA) and Abcam (UK), respectively. Mouse monoclonal antibody GAPDH was obtained from Wuhan Boster Corp. (China). Other reagents used were of the purest grade available.

Cepharanthine was purchased from Shiji-Aoke (Beijing, China), cell counting kit-8 was bought from APExBio (Houston, TX, United States), DMEM, penicillin, streptomycin, fetal bovine serum (FBS) and PBS were obtained from Gibco Life Technologies (Grand Island, NY, United States). Anti-GAPDH, P62 and anti-rabbit IgG HRP-linked antibody were obtained from Cell Signaling Technology, Inc., (Beverly, MA, United States). Cathepsin B antibody was purchased from the Santa Cruz Biotechnology (Santa Cruz, CA, United States). Primary antibodies against LC3 were obtained from Abcam (Cambridge, MA, United States), anti-rabbit HRP-conjugated secondary antibody and anti-mouse HRP-conjugated secondary antibody were purchased from Cell Signaling Technology (Boston, MA, United States).