The overall schematic of the assay is shown in Fig. 2 and Supplementary Fig. S1 . The paper microfluidic chip’s channel layout was designed using SolidWorks version 2020 (Dassault Systèmes, Vèlizy-Villacoublay, France) and wax-printed using ColorQube 8580 (Xerox Corporation, Rochester, NY, USA) on paper. Each channel is dumbbell-shaped (Fig. 2 ), where one end is used for introducing the solutions, and the other end acts as an absorbent pad. There are four channels in each chip (Supplementary Fig. S2 ), enabling four different assays with four different peptide-conjugated particles in a single run. Two nitrocellulose paper types were evaluated in this study to check the pape type dependency: Prima 40 from GE Healthcare (MA, USA) and CN95 from Sartorius (Goettingen, Germany). Capillary flow rates are 35–50 s over 40 mm for Prima 40 and 65–115 s over 40 mm for CN95, as reported by the manufacturers. Thicknesses are 180–220 μm for Prima 40 and 240–270 μm for CN95. FF 80HP Plus from GE Healthcare (MA, USA) was also evaluated additionally. Capillary flow rates are 60–100, thicknesses are 200 μm, as reported by the manufacturer.
Ff 80hp plus
Manufactured by GE Healthcare
Sourced in United States
The FF 80HP Plus is a compact, high-performance centrifuge from GE Healthcare designed for a wide range of laboratory applications. It features a robust and reliable motor, accommodating rotors for various sample volumes and tube sizes. The centrifuge provides precise speed and time controls to ensure consistent and reproducible results.
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Lab products found in correlation
3 protocols using ff 80hp plus
1
Paper-Based Microfluidic Chip for Multiplex Peptide Assays
2
Paper-based Microfluidic Chip Fabrication
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The paper-based microfluidic chips were printed the same way as in the previous work [8 , 10 (link)]. ColorQube 8580 wax printer (Xerox, Norwalk, CT, USA) was used to print the channel designs, followed by heating on a hot plate to melt the printed wax through the depth of a paper. A single chip contained four dumbbell-shaped channels for conducting four different assays, where the dumbbell shapes at both ends acted as the adsorbent pads. Nitrocellulose paper (FF80 HP Plus from GE Healthcare, Chicago, IL, USA) was used, whose capillary rise was 60–100 s per 4 cm, and the total caliper was 200 μm, according to the manufacturer.
3
Paper Microfluidic Chip Design and Fabrication
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The paper microfluidic chip was designed in SolidWorks 2020 software (Dassault Systèmes, Vélizy-Villacoublay, France) and wax printed on nitrocellulose paper using ColorCube 8550 (Xerox, Norwalk, CT, USA) as described previously57 (link). Each chip contains four flow channels that measure 21 mm long and 2.4 mm wide, with larger square-shaped loading pads at each end. The nitrocellulose paper (FF80HP Plus; GE Healthcare, MA, USA) had a capillary flow rate of 60–100 s over 40 mm and a thickness of 200 μm.
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