Stg 3008

Manufactured by MultiSciences Biotech

Sourced in Germany

The STG-3008 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 3,000 RPM and can accommodate a variety of sample tube sizes. The centrifuge is equipped with a digital speed display and timer for precise control over the separation process.

Automatically generated - may contain errors

Lab products found in correlation

Epr 800, by Millar (4 mentions) Labchart, by ADInstruments (1 mentions) Ecgauto2, by EMKA Technologies (1 mentions) Powerlab 16 30, by ADInstruments (1 mentions) Labchart pro, by ADInstruments (1 mentions)

7 protocols using stg 3008

Cardiac Electrophysiology Protocols in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Simultaneous atrial and ventricular pacing and recording were performed via 1.1F octapolar catheter (EPR-800, Millar) inserted via a jugular vein in mice anesthetized with isoflurane (2–3%). The standard pacing protocols^{11 (link)–13 (link)} were programmed in the multichannel stimulator STG 3008 (Multichannel systems), which was analyzed by LabChart (ADInstruments). Sinus nodal function was evaluated by measuring sinus node recovery time (SNRT) at three pacing drive rates (150, 120 and 100 ms) and corrected SNRT (SNRT minus sinus cycle length, SCL). AV conduction properties were assessed with rapid atrial pacing at rates with a minimum pacing cycle length of 50 ms (up to 1,200 bpm). The minimum cycle length maintaining 1:1 AV conduction, the Wenckebach paced cycle length, and the maximum paced cycle length causing 2:1 AV block were determined. AV nodal effective refractory periods were determined by the maximum coupling interval causing AV block at the two pacing drive rates (150 and 120 ms). Right ventricular effective refractory periods were determined by the maximum coupling interval that failed to stimulate ventricles at the two pacing drive rates (150 and 120 ms). Right ventricular burst pacing was performed at rates with a pacing cycle length of 150 to 50 ms (400-1,200 bpm) to assess retrograde ventriculo-atrial conduction.

Chowdhury R., Ashraf H., Melanson M., Tanada Y., Nguyen M., Silberbach G.M., Wakimoto H., Benson D.W., Anderson R.H, & Kasahara H. (2015). A Mouse Model of Human Congenital Heart Disease: Progressive Atrioventricular Block Induced by a Heterozygous Nkx2-5 Homeodomain Missense Mutation. Circulation. Arrhythmia and electrophysiology, 8(5), 1255-1264.

+ Open protocol

+ Expand

Intracardiac Electrophysiology in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized with isoflurane and placed on a heated pad to maintain body temperature at 37°C. A 1.1 French octapolar electrode catheter (EPR-800, Millar Instruments, Houston, TX) was advanced through the right jugular vein into the right atrium and right ventricle. Programmed electrical stimulation was performed using 2-ms current pulses delivered by an external stimulator (STG3008, Multichannel Systems, Reutlingen, Germany). Surface 6-lead ECG was recorded and monitored throughout the experimental procedures. The 8-lead intracardiac electrograms were recorded and analyzed with a computer-based data acquisition system (IOX-2 acquisition software and ecgAUTO2 software, Emka Technologies, VA, USA).
Right atrial overdrive pacing protocols of either S1S2 or S1S1 stimulus were used to determine atrial effective refractory period (AERP),, Wenckebach point, 2:1 atrioventricular block point or atrial tachycardia, respectively. Right ventricular overdrive pacing protocols of either S1S2 or S1S1 stimulus were performed to determine ventricular effective refractory period (VERP) or inducibility of ventricular tachycardia, respectively..

Yu Y., Ye L., Li Y.G., Burkin D.J, & Duan D.D. (2016). Heart-Specific Overexpression of the Human Short CLC-3 Chloride Channel Isoform Limits Myocardial Ischemia-induced ERP and QT Prolongation. International journal of cardiology, 214, 218-224.

+ Open protocol

+ Expand

Electrophysiological Assessment of Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PES technique was performed 1–2 weeks after BUN levels were measured (29 (link)). Mice were anesthetized using 1.5%–2.0% isoflurane in 100% O₂. A 1.1 F octapolar catheter (EPR-100, Millar Instruments) was inserted into the heart by cannulating the right jugular vein. Bipolar RA pacing was administered via an external stimulator (STG-3008, MultiChannel Systems). Electrophysiological data for SNRT and AVERP were obtained using standard clinical pacing protocols. AF susceptibility was determined using a rapid atrial burst pacing protocol as described previously (25 (link), 29 (link)). The experimenter was blinded to the mouse genotype and/or treatment group.

Song J., Navarro-Garcia J.A., Wu J., Saljic A., Abu-Taha I., Li L., Lahiri S.K., Keefe J.A., Aguilar-Sanchez Y., Moore O.M., Yuan Y., Wang X., Kamler M., Mitch W.E., Ruiz-Hurtado G., Hu Z., Thomas S.S., Dobrev D., Wehrens X.H, & Li N. (2023). Chronic kidney disease promotes atrial fibrillation via inflammasome pathway activation. The Journal of Clinical Investigation, 133(19), e167517.

+ Open protocol

+ Expand

Electrophysiological Evaluation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized with isoflurane and kept on a warm pad as mentioned above. A baseline surface ECG (lead II) was acquired using a multichannel data‐acquisition system (Powerlab 16/30; AD Instruments, Colorado Springs, CO). Data were stored using Labchart Pro software version 7 (AD Instruments). QT intervals were heart rate corrected (QTc) using Mitchell’s formula.²¹ Afterward, a standard clinical protocol was used to determine electrophysiological parameters. In brief, right atrial or ventricular pacing was performed using 2‐millisecond current pulses delivered by an external stimulator (STG‐3008; Multi Channel Systems). Inducibility of ventricular arrhythmias was tested by decremental burst pacing. Burst pacing started at a 40‐millisecond cycle length, decreasing by 2 milliseconds every 2 seconds to a cycle length of 20 milliseconds. Burst pacing was repeated 1 minute after the previous burst concluded or the termination of arrhythmias. Burst pacing was performed for a total of 5 times for ventricular stimulation, respectively. Ventricular tachycardia was defined as rapid ventricular potentials that occurred independent from atrial potentials and displayed altered QRS morphology. If ventricular tachycardia was sustained for >1 second, it was considered as relevant.

Islam M.M., Tarnowski D., Zhang M., Trum M., Lebek S., Mustroph J., Daniel H., Moellencamp J., Pabel S., Sossalla S., El‐Armouche A., Nikolaev V.O., Shah A.M., Eaton P., Maier L.S., Sag C.M, & Wagner S. (2021). Enhanced Heart Failure in Redox‐Dead Cys17Ser PKARIα Knock‐In Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(19), e021985.

+ Open protocol

+ Expand

Intra-esophageal Pacing for Atrial Arrhythmia Induction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Intra-esophageal pacing was performed by placing in the esophagus, close to the left atrium, a 1.1-Fr octapolar catheter (EPR-800, Millar Instruments, Houston, Texas) connected to an external stimulator (STG-3008, MultiChannel Systems, Reutlingen, Germany). A computerized data acquisition system (EMKA Technologies, Falls Church, VA) was used to record a 3-lead surface ECG, and up to 4 intra-esophageal bipolar electrocardiograms. Inducibility of atrial arrhythmias was tested by applying a series of 2-second bursts. The first 2-second burst had a cycle length (CL) of 40 ms; then the CL was progressively decreased by 2 ms in each successive burst until reaching 10 ms. AF was defined as a period of rapid irregular atrial rhythm lasting at least 1 sec.

Xie W., Santulli G., Reiken S.R., Yuan Q., Osborne B.W., Chen B.X, & Marks A.R. (2015). Mitochondrial oxidative stress promotes atrial fibrillation. Scientific Reports, 5, 11427.

+ Open protocol

+ Expand

In Vivo Electrophysiology in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

In vivo electrophysiology studies were performed in knockout and wild-type mice of both sexes, as per prior established protocols [15 (link), 37 (link)]. Atrial and ventricular intracardiac electrograms were recorded simultaneously using a 1.1F octapolar catheter (EPR-800, Millar Instruments, Houston, TX, USA) inserted via the right jugular vein. Surface ECG and intracardiac electrophysiology parameters were assessed at baseline. Right atrial pacing was performed using 2-ms current pulses at 800 µA delivered by an external stimulator (STG-3008, Multi Channel Systems, Reutlingen, Germany). AF inducibility was determined using an overdriving pacing protocol, and AF was defined as the occurrence of rapid and fragmented atrial electrograms with irregular AV-nodal conduction and ventricular rhythm for at least 1 s. To be counted as AF positive, a mouse had to exhibit AF in response to at least two out of three pacing trials. For Kcna1-null mice, the mean AF duration was determined by calculating the average elapsed time of all observed AF episodes. The stimulation protocols used for intracardiac burst pacing are summarized in Supplemental Table S1.

Glasscock E., Voigt N., McCauley M.D., Sun Q., Li N., Chiang D.Y., Zhou X.B., Molina C.E., Thomas D., Schmidt C., Skapura D.G., Noebels J.L., Dobrev D, & Wehrens X.H. (2015). Expression and function of Kv1.1 potassium channels in human atria from patients with atrial fibrillation. Basic research in cardiology, 110(5), 505.

+ Open protocol

+ Expand

Atrial Fibrillation Induction in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

AF induction in a rat model was based on a previously described study. 15 (link) In brief, for the AF model in isolated hearts, the Langendorff perfusion system and ECG recording system were established following the previous protocol. 16 A 1.8F catheter (Millar Instruments, TX) was inserted to pace the right atrium. When a stable ECG appeared, AF was induced by a burst stimulation (10 Hz lasting 1000 ms). AF was considered inducible if the burst stimulus produced a period of irregular atrial electrograms (>800 beats per minute) with irregular ventricular response and lasted for at least 1000 ms. 17 In vivo electrophysiology in rats was conducted as previously described. 15 (link) The rats were anesthetized using 1.5% isoflurane. A catheter was inserted through the jugular vein to pace the right atrium. Right atrial pacing was performed using 1-ms current pulses delivered by an external stimulator (STG-3008, Multi-Channel Systems, Germany). The atrial effective refractory period was determined by applying a series of atrial-pacing trains at a fixed basic cycle length of 140 ms (S1) with an S2 premature stimulus. To assess AF inducibility, S1-S2-S3 premature stimuli were applied. Additional details are provided in the Data Supplement.

Geng L., Wang S., Zhang F., Xiong K., Huang J., Zhao T., Shi D., Lv F., Li L., Liang D., Cui Y., Liu Y., Xie D, & Chen Y.H. (2019). SNX17 (Sorting Nexin 17) Mediates Atrial Fibrillation Onset Through Endocytic Trafficking of the Kv1.5 (Potassium Voltage-Gated Channel Subfamily A Member 5) Channel. Circulation. Arrhythmia and electrophysiology, 12(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!