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Sigma kit ht15

Manufactured by Merck Group
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The Sigma kit HT15 is a laboratory equipment product used for various analytical and research applications. It provides a set of standardized tools and reagents to facilitate specific experimental procedures. The core function of this kit is to enable consistent and reliable data collection in a laboratory setting. Further details on its intended use are not available.

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Lab products found in correlation

Aperio at2, by Leica (1 mentions) Axioplan 2 microscope system, by Zeiss (1 mentions)

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HT15 kit (5 citations) Sigma kits (5 citations)

2 protocols using sigma kit ht15

1

Quantifying Collagen in Muscle Tissue

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Cryostat muscle sections (10 µm) were stained with Masson’s trichrome stain to visualize connective tissue and muscle fibers in pink and collagen in blue (Sigma kit HT15; Sigma‐Aldrich) and then observed using a scanner Leica Aperio® AT2 (Leica Microsystems). Collagen (stained in blue) was quantified using the Aperio Image Analysis IHC, Leica.
de Zélicourt A., Fayssoil A., Dakouane‐Giudicelli M., De Jesus I., Karoui A., Zarrouki F., Lefebvre F., Mansart A., Launay J., Piquereau J., Tarragó M.G., Bonay M., Forand A., Moog S., Piétri‐Rouxel F., Brisebard E., Chini C.C., Kashyap S., Fogarty M.J., Sieck G.C., Mericskay M., Chini E.N., Gomez A.M., Cancela J, & de la Porte S. (2022). CD38‐NADase is a new major contributor to Duchenne muscular dystrophic phenotype. EMBO Molecular Medicine, 14(5), e12860.
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2

Histological Analysis of Skeletal Muscle

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Muscle samples were frozen in liquid nitrogen-chilled isopentane, and stored at −80°C. Transverse and longitudinal extensor digitorum longus (EDL), tibialis anterior (TA), soleus (SOL), diaphragm (DIA) and heart cryosections (10 μm) were stained with Haematoxylin and Eosin solution (H&E), Masson's trichrome (MT) and Alizarin red (AR). The Axioplan 2 Microscope System (Carl Zeiss, Germany) was used to obtain the images. The proportion of centrally nucleated fibres was determined by analysing the H&E images. Areas of necrosis were quantified based on the DMD_M.1.2.007 MDC1A_M.1.2.004 TREAT-NMD SOPS and performed with the Fiji ImageJ 1.49i software (73 ) on the whole EDL, SOL and DIA sections. Fibrosis of the whole DIA sections was observed using Masson's trichrome staining to visualize connective tissue and muscle fibres (Sigma kit HT15; SigmaAldrich) and quantified using a previously published macro (74 (link)) with the Fiji ImageJ 1.49i software.
Guiraud S., Squire S.E., Edwards B., Chen H., Burns D.T., Shah N., Babbs A., Davies S.G., Wynne G.M., Russell A.J., Elsey D., Wilson F.X., Tinsley J.M, & Davies K.E. (2015). Second-generation compound for the modulation of utrophin in the therapy of DMD. Human Molecular Genetics, 24(15), 4212-4224.
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