L ascorbic acid 2 phosphate sequimagnesium salt

Manufactured by Merck Group

L-ascorbic acid 2-phosphate sequimagnesium salt is a chemical compound commonly used as a laboratory reagent. It is a stable derivative of ascorbic acid (vitamin C). The compound serves as a source of ascorbic acid in various applications that require a stable and soluble form of the vitamin.

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Lab products found in correlation

Keratinocyte sfm, by Thermo Fisher Scientific (2 mentions) Egf bpe, by Thermo Fisher Scientific (2 mentions) N acetyl l cysteine, by Merck Group (2 mentions)

Close spelling variants of this product

Ascorbic acid (4565 citations) L-ascorbic acid (1638 citations) L-ascorbic acid 2-phosphate (383 citations) Ascorbic acid 2-phosphate (326 citations) Ascorbic-2-phosphate (74 citations) L-ascorbic acid phosphate (28 citations) Ascorbic acid phosphate (24 citations) Phosphate salts (4 citations) L-ascorbic acid 2-phosphate sequimagnesium (2 citations)

2 protocols using l ascorbic acid 2 phosphate sequimagnesium salt

Isolation and Culture of Adipose-Derived Stem Cells

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Example 1

Isolation and Culture of hADSCs

After obtaining informed consent from all the patients and approval from the Kaohsiung Medical university hospital ethics committee, leftover subcutaneous adipose tissue was acquired from patients undergoing orthopedic surgery. The hADSCs were isolated from human subcutaneous adipose tissue following the previously described method (Fehrer and Lepperdinger, Exp Gerontol, 2005, 40:926-930). The isolated hADSCs were cultured and expanded at 37 C under 5% CO₂in K-NAC medium containing Keratinocyte-SFM (Gibco BRL, Rockville, Md.) supplemented with the EGF-BPE (Gibco BRL, Rockville, Md.), N-acetyl-L-cysteine, L-ascorbic acid 2-phosphate sequimagnesium salt (Sigma, St. Louis, Mo.) and 5% FBS (Fehrer and Lepperdinger, Exp Gerontol, 2005, 40:926-930).

US09889225B2. Method for bone formation by administering poly(lactic-co-glycolic acid) cross-linked alendronate (2018-02-13). KAOHSIUNG MEDICAL UNIVERSITY [TW]. Inventors: Mei-Ling Ho [TW], Je-Ken Chang [TW], Rajalakshmanan Eswaramoorthy [TW], Shun-Cheng Wu [TW], Yao-Hsien Wang [TW].

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Isolation and Expansion of Rabbit Adipose-Derived Stem Cells

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The rADSCs were isolated from 3-month-old New Zealand white rabbit (National Laboratory Center, Taipei, Taiwan) subcutaneous adipose tissues following previously described methods^{5 (link),16 (link),17 (link)} with the approval of the Kaohsiung Medical University Animal Care and Use Committee, and all methods were performed in accordance with the relevant guidelines and regulations. Briefly, the isolated rADSCs were cultured and expanded at 37 °C under 5% CO₂ in selective K-NAC medium which containing Keratinocyte-SFM (Gibco BRL, Rockville, MD), EGF-BPE (Gibco BRL, Rockville, MD), N-acetyl-L-cysteine, L-ascorbic acid 2-phosphate sequimagnesium salt (Sigma, St. Louis, MO) and 5% FBS. This medium can maintain the characterization of pluripotent stem cells and self-renewal properties of ADSCs^{5 (link),16 (link),17 (link)}.

Wang C.Z., Eswaramoorthy R., Lin T.H., Chen C.H., Fu Y.C., Wang C.K., Wu S.C., Wang G.J., Chang J.K, & Ho M.L. (2018). Enhancement of chondrogenesis of adipose-derived stem cells in HA-PNIPAAm-CL hydrogel for cartilage regeneration in rabbits. Scientific Reports, 8, 10526.

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