Dc661

Manufactured by Selleck Chemicals

Sourced in United States

The DC661 is a laboratory instrument used for the detection and analysis of chemical compounds. It utilizes digital signal processing technology to provide precise measurements and data processing capabilities. The core function of the DC661 is to facilitate accurate and reliable chemical analysis in a laboratory setting.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Chloroquine, by Merck Group (1 mentions) N acetyl l cysteine, by Selleck Chemicals (1 mentions) Sulforaphane, by Selleck Chemicals (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Water soluble cholesterol, by Merck Group (1 mentions) Lysosensor green dnd 189, by Thermo Fisher Scientific (1 mentions) Simvastatin, by Cayman Chemical (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Fumonisin b1, by Cayman Chemical (1 mentions) Myriocin, by Cayman Chemical (1 mentions) Blt 1, by MedChemExpress (1 mentions) Sbi 0206965, by Cayman Chemical (1 mentions) Sar 405, by Cayman Chemical (1 mentions)

3 protocols using dc661

Antibody-Based Cellular Assay Reagents

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Antibodies used in this study were listed in Supplementary Table S1. ECL-plus kit was from NCM Biotech in China. The Chloroquine (CQ) was purchased from Sigma–Aldrich Chemical Company (St. Louis, MO, USA). And CC90003, DC661, N-acetyl-L-cysteine (NAC), Sulforaphane (SFN) were obtained from Selleck Chemicals (Houston, TX, USA). Lipofectamine 2000 was purchased from Invitrogen (USA).

Mi W., Wang C., Luo G., Li J., Zhang Y., Jiang M., Zhang C., Liu N., Jiang X., Yang G., Zhang L., Zhang G., Zhang Y, & Fu Y. (2021). Targeting ERK induced cell death and p53/ROS-dependent protective autophagy in colorectal cancer. Cell Death Discovery, 7, 375.

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Fluorescent Calcium Imaging with HCQ

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Chemicals purchased included HCQ Sulfate (Spectrum Chemicals, 747-36-4) and DC661 (Selleckchem, S8808). Fluo-4, AM (Thermo Fisher Scientific, F14201) was used to stain the cells for calcium as per the manufacturer’s instructions. A list of antibodies and inhibitors is provided in Supplemental Table 1 and Supplemental Table 2.

Bhardwaj M., Lee J.J., Versace A.M., Harper S.L., Goldman A.R., Crissey M.A., Jain V., Singh M.P., Vernon M., Aplin A.E., Lee S., Morita M., Winkler J.D., Liu Q., Speicher D.W, & Amaravadi R.K. (2023). Lysosomal lipid peroxidation regulates tumor immunity. The Journal of Clinical Investigation, 133(8), e164596.

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Comprehensive Chemical Library for Cell Experiments

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Chemicals purchased included Hydroxychloroquine (HCQ) Sulfate (Spectrum Chemicals, 747–36-4); DC661 (Selleckchem, S8808); Methyl-Beta-Cyclodextrin (332615, Sigma); Cholesterol-Water Soluble (C4951, Sigma); Simvastatin (10010344, Cayman Chemical); BLT-1 (HY-116767, MedChemExpress); Myriocin (63150–5, Cayman Chemical); Fumonisin B1 (62580–1, Cayman Chemical); Siramesine hydrochloride (6740, Tocris Bioscience); Genz-123346 (BioVision, B2286); Eliglustat hemitartarate (HY-14885A, MedChemExpress LLC); Eliglustat hemitartarate (21487, Cayman Chemical); SBI-0206965 (18477, Cayman Chemical); SAR-405 (16979, Cayman Chemical) and LysoSensor^™ Green DND-189 (Thermo Fisher; L7535). ST1074 was synthesized by Holger Stark and Aleksandra Zivkovic (21 (link)).

Jain V., Harper S.L., Versace A.M., Fingerman D., Brown G.S., Bhardwaj M., Crissey M.A., Goldman A.R., Ruthel G., Liu Q., Zivkovic A., Stark H., Herlyn M., Gimotty P.A., Speicher D.W, & Amaravadi R.K. (2023). Targeting UGCG overcomes resistance to lysosomal autophagy inhibition. Cancer discovery, 13(2), 454-473.

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