Sc 13073

Cells and tissues were lysed by RIPA buffer and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were transferred to nitrocellulose membranes (Pierce, Rockford, IL, USA). The membranes were blocked with 5% nonfat dry milk in TBST. Primary antibodies were: SMAD7 (MAB2029, R&D Systems, Minneapolis, MN, USA), P21 (SAB4500065, Sigma Aldrich), pSMAD2 (Ser465/467), pSMAD1/3, BCL-2, BCL-XL, pSTAT3, pJNK, pc-JUN, MCL-1, cleaved caspase-3 and Actin (3101, 9520,2870, 2762, 9145, 4668, 3270,5453,9661 and 3700, Cell Signaling Technology), c-MYC, pIκBα, IL-6, TGF-β1, pERK, CyclinD1, pP38 and VEGF (sc-40, sc-1265, sc-8404, sc-130348, sc-13073, sc-753, sc-7973, and sc-507, Santa Cruz Biotechnology). Secondary antibodies were: horseradish peroxidase-linked anti-rabbit and anti-mouse antibodies (Santa Cruz Biotechnology). The membranes were developed using Supersignal Ultra (Pierce).

Breifly, 4-PBA and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Daclatasvir (DAV) was purchased from MedChem Express (MedChem Express, Monmouth Junction, NJ, USA). Antibodies used in this study include mouse anti-NS5A; sc-65458, mouse anti-NS3; sc-69938, mouse anti-a-actinin; sc-17829 rabbit anti-hVAP-33; sc-98890, anti-grp78;sc-13968, rabbit anti-PERK; sc-13073, rabbit anti-IRE1a; sc-20790, mouse anti-actin; sc-8432 (Santa cruz, Dallas, TX, USA), rabbit anti-hepcidin; ab75883, rabbit anti-H3K9ac; ab10812, rabbit anti-H3K27ac; ab4729, mouse anti-RNA pol III; ab817, rabbit anti-CypB; ab16045, (abCAM, Canbridge, UK), rabbit anti-OSBP; #11096-1-AP (proteintech, Rosemont, IL, USA), rabbit anti-VAP-B; NBP1-89112 (NOVUS, Centennial, CO, USA), mouse anti-OCH1E5; MBS370077, rabbit anti-NS5A (For Immunohistochemistry); MBS485095 (mybiosource, San Diego, CA, USA). Secondary antibodies used are goat anti-mouse IgG; M32607, goat anti-rabbit IgG; 31460, Alexa Fluor 488 goat anti-rabbit IgG; A-11034, Alexa Fluor 488 goat anti-mouse IgG; A-11001, Alexa Fluor 594 goat anti-mouse IgG; A-11005, Alexa Flour 594 goat anti-rabbit IgG; A-11012 (Invitrogen, Waltham, MA, USA).