Rat anti cdh1

Manufactured by Santa Cruz Biotechnology

The Rat anti-CDH1 is a laboratory reagent used for the detection and analysis of the CDH1 protein. CDH1, also known as E-cadherin, is a cell-cell adhesion molecule that plays a crucial role in the maintenance of epithelial cell structure and function. The Rat anti-CDH1 antibody can be used in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and quantify the expression of CDH1 in biological samples.

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2 protocols using rat anti cdh1

Immunofluorescence Staining of Lung Cells

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The following antibodies were used: Mouse anti-αSMA-Cy3 (1:1000, Sigma-Aldrich, C6198); Rat anti-CDH1 (1:500, Santa Cruz, sc-59778); Goat anti-KCNJ13 (1:50, Santa Cruz, C19); Rabbit anti-SOX9 (1:400, Millipore, AB5535); Sheep anti-GM130 (1:50, R&D systems, AF8199); Rabbit anti-Ki67 (1:400, Cell Signaling Technologies, #9027); Rabbit anti Cleaved Caspase-3 (1:600, Cell Signaling Technologies, #9661); Rabbit anti KRT5 (1:1000, Abcam, ab53121); Goat anti-CC10 (1:200, Santa Cruz, T-18); Rabbit anti-NKX2.1 (1:400, Santa Cruz, H-190); Rabbit anti-SFTPC (1:400, Millipore, AB3786); Mouse anti-PCNA (1:400, Santa Cruz, sc-56); Rabbit anti-PH3 (1:400, Millipore, 06-570); Rabbit anti-p-AKT^ser473 (1:200, Cell Signaling Technologies, #4060); Rabbit anti-AKT (1:2000 Cell Signaling Technologies, #9272), and Rabbit anti-GAPDH (1:3000, Cell Signaling Technologies, #2118).

Yin W., Kim H.T., Wang S., Gunawan F., Wang L., Kishimoto K., Zhong H., Roman D., Preussner J., Guenther S., Graef V., Buettner C., Grohmann B., Looso M., Morimoto M., Mardon G., Offermanns S, & Stainier D.Y. (2018). The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis. Nature Communications, 9, 2815.

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Comprehensive Immunohistochemical Profiling of Lung Cells

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The following antibodies were used: rat anti-CDH1 (1:200, Santa Cruz, sc-59778); mouse anti-CDH1 (1:100, BD Biosciences, 560062); rabbit anti cleaved caspase-3 (1:600, Cell Signaling Technologies, #9661); goat anti-SCGB1A1 (1:200, Santa Cruz, T-18); rabbit anti-NKX2.1 (1:400, Santa Cruz, H-190); mouse anti-acetyl-α tubulin (1:2000, Sigma, MABT868); mouse anti-FOXJ1 (1:400, Thermo Fisher Scientific, 14-9965-82); rabbit anti-MUC5AC (1:400, Santa Cruz, H-160); rabbit anti-MUC5B (1:400, Novus Biologicals, NBP1-92151); mouse anti-BrdU (1:400, Thermo Fisher Scientific, B35141); hamster anti-PDPN (1:20, DSHB, 8.1.1); rat anti-SCGB3A1 (1:200, R&D Systems, MAB2954); rat anti-SCGB3A2 (1:200, R&D Systems, MAB3465); rabbit anti-Ki67 (1:400, Thermo Fisher Scientific, PA5-19462); goat anti-NGFR (1:200, Santa Cruz, C-20); mouse anti-α-SMA-Cy3 (1:1000, Sigma-Aldrich, C6198); rabbit anti-SOX9 (1:400, Millipore, AB5535MA); goat anti-SOX9 (1:500, R&D Systems, AF3075); rat anti-Cd140a (1:100, Biolegend, 135901); rabbit anti-RFX3 (1:500, Sigma, HPA035689); mouse anti-TRP63 (1:500, Abcam, ab735); rabbit anti-TRP63 (1:100, Cell Signaling Technology, 13109S) and chicken anti-KRT5 (1:400, Biosite, 905901).

Yin W., Liontos A., Koepke J., Ghoul M., Mazzocchi L., Liu X., Lu C., Wu H., Fysikopoulos A., Sountoulidis A., Seeger W., Ruppert C., Günther A., Stainier D.Y, & Samakovlis C. (2022). An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea. Development (Cambridge, England), 149(3), dev199804.

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