Male and female C57BL/6N mice were obtained from Charles River Laboratories (Wilmington, MA, USA) and allowed to acclimate for at least 1 week prior to the start of the experiments. Mice were housed in pairs (same sex and age) in polycarbonate cages (28 × 17 × 12 cm) with bed-o’cobs 1/4 inch bedding and maintained on a 12-h light dark schedule (lights on at 8:00 h). The temperature was maintained at 21 ± 2°C and mice had ad libitum access to water and rodent chow (Lab Diet #5012; PMI Nutrition International LLC, Brentwood, MO, USA). The stage of the estrous cycle was not determined in the female mice. All procedures were approved by the Institutional Animal Care and Use Committee of Columbia University.
Lab diet 5012
Manufactured by Land O'Lakes
Sourced in United States
Lab Diet #5012 is a specialized laboratory animal diet formulated to meet the nutritional requirements of laboratory animals. It provides a complete and balanced source of nutrients essential for the growth and maintenance of laboratory animals.
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Lab products found in correlation
5 protocols using lab diet 5012
1
Acclimation of C57BL/6N Mice
2
Cardioprotective Effects of CS and CZ
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Male Wistar rats of 300–350 g were used. They were provided by the Laboratory Animal Care of the National Institute of Cardiology “Ignacio Chávez” in México. All procedures for handling animals were approved by the Institutional Ethics Committee and in accordance with the National Rules for the care and handling of experimental animals (SAGARPA, NOM-062-ZOO-1999). The animals were kept under optimal conditions of temperature and light (12 h light/dark) with a standard diet (Lab diet 5012, PMI Nutrition International, Richmond, IN, USA) and water ad libitum. The experimental animals were randomly grouped as follows: 1.—Control; 2.—CS treatment (final dose of 20 mg/kg which was distributed over 4 days); 3.—CZ treatment (final dose of 24 mg/kg distributed over 4 days) and 4.- CZ+CS treatment for 4 days. The CZ was applied first and, an hour later, the CS was injected. CS was applied at a lower dose (20 mg/kg) than that reported by Zhou et al. and by Szabados et al. [18 (link),23 (link)] to avoid deletion of nerve endings and also because we performed a curve dose response in previous experiments with CS and CZ in isolated hearts [24 (link)]. The application of the drugs was through a s.c. injection.
3
Zucker Rat Model for Obesity Research
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All animal experiments were conducted in accordance with a protocol approved by the Institutional Animal Care and Use Committee (IACUC) at Rutgers, The State University of New Jersey. Male Zucker rats (ZUC-LEAN (control) and ZUC-FA/FA (obese)) were purchased from Charles River (Wilmington, MA). Upon arrival, 5-week-old animals were housed in pairs in solid bottom cages on a 12/12 h dark/light cycle and provided with a standard rat diet (LabDiet 5012, PMI Nutrition International) and water ad libitum. Various measures of body size were performed throughout the study, as described below. In IV dosing groups, the right jugular vein was cannulated (under isoflurane anesthesia) using PE-50 tubing to allow IV infusion. After surgery, animals were housed individually in cages and allowed to recover for 72 h; SC meloxicam/carprofen and intradermal bupivacaine analgesia were provided.
4
Sprague-Dawley Rat Breeding and Housing
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Male and female Sprague-Dawley rats obtained from our breeding colony at Barnard College were used for these experiments. The breeding stock was originally obtained from Charles River Laboratories (Wilmington, MA). On the day of birth, litters were culled to twelve pups, trying to maintain an even number of males and females. Litters were weaned at 21 days of age and housed in same-sex pairs. No more than two males and two females from a single litter were used to compose each experimental group. Before and after weaning, animals were housed in clear polycarbonate cages with bed-o’ cobs ¼ inch bedding, had ad libitum access to food (Lab Diet #5012; PMI Nutrition International, LLC; Brentwood, MO) and water, and were maintained on a 12:12-h light/dark schedule (lights on at 0900h). Until the day of tissue collections, animals were left undisturbed throughout the experiment, expect for routine cage maintenance and care. All procedures were carried out in accordance with the guidelines established by the NIH Guide for the Care and Use of Laboratory Animals and the Animal Experimentation Guidelines from the Columbia University Institute of Comparative Medicine. All procedures were approved by the Institutional Animal Care and Use Committee of Columbia University.
5
Wistar Rat Handling and Housing
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All procedures in handling animals were approved by the Institutional Ethics Committee according to the National Rules for the care and handling of experimental animals (SAGARPA, NOM-062-ZOO-1999) [20 ]. Male Wistar rats of 300 to 350 g were kept under standard conditions of temperature and light (12 h light/dark) with a standard diet (Lab diet 5012, PMI Nutrition International, Richmond, IN, USA) and water ad libitum.
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