Folin ciocalteus s phenol reagent

Folin-Ciocalteus’s phenol reagent was procured from Beijing Solarbio Co. Ltd, PR China. 1, 1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azino-bis (3-ethyl-benzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 2, 4, 6-Tripyridyl-s-triazine (TPTZ), 6-Hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (Trolox), and seven chemical standards (rutin, quercetin, kaempferol, gallic acid, chlorogenic acid, protocatechuic acid, ferulic acid) were provided by Sigma-Aldrich Co., St. Louis, Missouri, USA. Acetic acid, chromatographic-scale methanol and acetonitrile were obtained from Tianjin Bodi Chemical Holding Co. Ltd., China. All solutions involved in this work were filtered with a 0.22 mm nylon filter before use. Analytical-grade reagents were dissolved using deionized water (18 MΩcm). T-400B high-speed multi-function pulverizer was acquired from Dingshuai Hardware Products Co., Ltd., Yongkang City, Zhejiang Province. R-1001VN rotary evaporator was provided from Changcheng Science and Industry Co., Ltd., Zhengzhou City, Henan Province. The HPLC was carried out by an Agilent Series 1260 liquid chromatograph equipped with a quaternary gradient pump system and variable-wavelength detector (VWD) system with a reversed-phase (RP) SB-C18 column (5 μm, 4.6 ×250 mm, Agilent Technologies Inc., USA). Data collection was performed using ChemStation (Agilent Technologies Inc., USA).

A HPLC analysis was conducted with an Agilent Series 1260 liquid chromatograph equipped with a quaternary gradient pump system and variable-wavelength detector system connected to a reversed-phase (RP) SB-C 18 column (5 μm, 4.6 × 250 mm, Agilent, USA). Data collection was performed using ChemStation software (Agilent, USA). Chromatograph-grade methanol and acetonitrile were purchased from Sigma-Aldrich Co. Ltd (St. Louis, Missouri, USA).
Folin-Ciocalteus's phenol reagent (Beijing Solarbio Co. Ltd, PR China), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethyl-benzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 2,4,6-Tripyridyl-s-triazine (TPTZ), and 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, Sigma-Aldrich Co., St. Louis, Missouri, USA), gallic acid, quercetin, and acetic acid (Tianjin Bodi Chemical Holding Co. Ltd, PR China) were used. All solutions were filtered through 0.22 mm nylon filters before use. Reagents were of analytical grade and dissolved in deionized water (18 MΩ cm).

HPLC analyses were conducted on an Agilent Series 1260 liquid chromatograph at ambient temperature. The instrument is comprised of a quaternary gradient pump, a variable wavelength detector system and a reversed-phase SB-C18 column (5 μm, 4.6×250 mm, Agilent, USA). Data acquisition was performed with ChemStation software (Agilent, USA).
Folin-Ciocalteus’s phenol reagent was purchased from Solarbio Co., Ltd (Beijing, China). 2,4,6-Tripyridyl-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) were purchased from the Sigma-Aldrich Co., St. Louis, USA. Standards including tannic acid, rutin, gallic acid, quercetin and kaempferol were obtained from the Chinese National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetic acid, sodium nitrite, HPLC grade methanol, sodium hydroxide and sodium carbonate were provided by the Tianjin Bodi Chemical Holding Co. Ltd (Tianjin, China), who also supplied the other chemicals of analytical grade. Deionized water (18 MΩ cm) served as the solvent for aqueous solutions. Stock solutions of all reagents employed here were prepared in methanol and diluted to the required concentration.