The antibodies that we used for the Western Blot assay are as follows: Primary antibody: anti-S100A11 (10237-1-AP) was purchased from Proteintech (USA), anti-GAPDH (P04406), anti-E-cadherin(#14472), anti-ERK (#4695T), anti-p-ERK (#4370T), anti-AKT (#5691S) and anti-p-AKT (#4060S) were from Cell Signaling Technology (Shanghai, China), anti-N-cadherin (ab76011) was from Abcam company (MA, United States); Secondary antibodies: anti-mouse IgG, HRP-linked antibody(#7076) and mouse anti-rabbit IgG mAb (HRP conjugate) were also purchased from Cell Signaling Technology (Shanghai, China). PrimScript RT reagent Kit with gDNA Eraser (RR047A) was from Takara (Japan), and SYBR Green PCR Master Mix was from Thermo Fisher Scientific (USA).
Anti n cadherin ab76011
Manufactured by Abcam
Sourced in China, United Kingdom, United States
Anti-N-cadherin (ab76011) is a primary antibody that recognizes the N-cadherin protein. N-cadherin is a cell adhesion molecule involved in cell-cell interactions. This antibody can be used in various applications to detect and study N-cadherin expression.
Automatically generated - may contain errors
Lab products found in correlation
Anti vimentin ab92547, by Abcam (2 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions)
Anti p akt 4060s, by Cell Signaling Technology (1 mentions)
Primscript rt reagent kit with gdna eraser, by Takara Bio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Anti iκbα ab32518, by Abcam (1 mentions)
Anti β catenin ab32572, by Abcam (1 mentions)
Anti mmp9 ab76003, by Abcam (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Tpc 1, by National Collection of Authenticated Cell Cultures (1 mentions)
Goat anti rabbit igg, by LI COR (1 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Anti β actin ab8226, by Abcam (1 mentions)
Anti snail ab180714, by Abcam (1 mentions)
Goat anti mouse igg antibodies, by LI COR (1 mentions)
Gw4869, by Merck Group (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Vimentin ab92547, by Abcam (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
4 protocols using anti n cadherin ab76011
1
Western Blot Assay for S100A11
2
Investigating FKBP51 Regulation of PTC Metastasis
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The human PTC cell lines K1 and TPC-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640, Dulbecco's modified Eagle's medium and fetal bovine serum (FBS) were purchased from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Anti-FKBP51 (ab46002) used in immunohistochemistry (IHC), anti-IκBα (ab32518), anti-N-cadherin (ab76011), anti-Vimentin (ab92547), anti-β-catenin (ab32572) and anti-MMP9 (ab76003) were purchased from Abcam (Cambridge, UK). Anti-p65 (sc-8008), anti-FKBP51 (sc-13983), anti-GAPDH (sc-47724), anti-α-tubulin (sc-53646), secondary antibody anti-mouse IgG-horseradish peroxidase (HRP; sc-516102) and anti-rabbit IgG-HRP (sc-2372) used in western blotting were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-TGF-β1 (3709) and anti-histone 3 (12164) was purchased from Cell Signaling Technology (Danvers, MA, USA). Ammonium pyrrolidine dithiocarbamate (PDTC; T3147) was purchased from Target Molecule Corp. (Wellesley Hills, MA, USA).
3
ADAM17 Regulation in Cell Signaling
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GW4869 was obtained from Sigma-Aldrich (St. Louis, MO, United States). Lipofectamine® 3,000 Transfection Reagent was purchased from Thermo Scientific (USA). RIPA lysis buffer was purchased from Beyotime (Jiangsu, China). Primary antibodies for immunoblot analysis included anti-ADAM17 (ab39163), anti-CD81 (ab79559), anti-E-cadherin (ab231303), anti-N-cadherin (ab76011), anti-vimentin (ab92547), anti-snail (ab180714), and anti-β-actin (ab8226) (Abcam, Cambridge, MA, United States). Goat anti-rabbit IgG and goat anti-mouse IgG antibodies were purchased from LI-COR (Lincoln, NE, United States).
4
Immunoblotting Antibodies for Cell Signaling
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Antibodies against FOXC1 (#8758), rabbit IgG (#7074), mouse IgG (#7076) and GAPDH (#5174) were obtained from Cell Signaling Technologies; Anti-SOX9 (ab3697), anti-MYC (ab32072), anti-RUNX1 (ab138377) anti-Snail (ab53519), anti-E-cadherin (ab133597), anti-N-cadherin (ab76011) and anti-Vimentin (ab92547) were from Abcam; Antibodies were used at the manufacturer’s recommended dilutions. THZ1 was obtained from MedChem Express and diluted in DMSO.
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