Ivis lumina 3 series hardware

Bioluminescent imaging was used as a surrogate measure for intracranial tumor size. Approximately 7 days post‐intracranial tumor cell delivery, mice were imaged using the IVIS imaging system (IVIS Lumina III Series Hardware, Perkin‐Elmer, Waltham). Each mouse received an intraperitoneal injection of D‐luciferin (Promega, Madison), and 6 min later, mice were anaesthetised with isoflurane inhalant (initial 4%, maintained at 2%). Mice were placed onto the platform in the IVIS system, and metal dividers were placed between the mice to reduce signal being detected in neighbouring mice. Anaesthetised mice were imaged over a maximum of 2 min, before removing mice and monitoring recovery. Tumor growth was monitored over time, and the experiment was repeated twice. Image analysis was performed using the Living Image Software (Perkin‐Elmer, Waltham, software version 4.7.2), and average radiance values (Radiance/cm/sec/sr) were plotted in GraphPad Prism.