Lenti pac hiv qrt pcr titration kit

Lentiviruses were produced in HEK293T cells using Lenti-Pac HIV Expression Packaging Kit, as per manufacturer’s guidelines (GeneCopoeia, Rockville, MD, USA; #LT001). Lentivirus titers were measured using Lenti-Pac HIV qRT-PCR Titration Kit (GeneCopoeia, #LT001) according to the manufacturer’s protocol. HFF cells were transduced with the different lentivirus preparations for 3 days in the culture medium supplemented with 5 µg/mL Polybrene (hexadimethrine bromide). Transduced cultures were either used immediately for downstream experiments or selected for 6 days using 2 µg/mL puromycin to generate stable cell lines. Plasmids, shRNA clones, and open reading frame expression clones were purchased from GeneCopoeia (Table S2).