Adiponectin

Manufactured by Linco Research

Sourced in United States

Adiponectin is a protein hormone that is involved in regulating glucose and fat metabolism. It is secreted by adipose tissue and plays a role in insulin sensitivity and energy homeostasis.

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Lab products found in correlation

Leptin, by Linco Research (4 mentions) Resistin, by Linco Research (3 mentions) Resistin, by BioVendor (1 mentions) Tnfrii, by Assaypro (1 mentions) Tnf α, by Merck Group (1 mentions) Glucagon, by Merck Group (1 mentions) β hydroxybutyrate, by Randox (1 mentions) Leptin, by BioVendor (1 mentions) Insulin, by Merck Group (1 mentions) Radioimmunoassay, by Merck Group (1 mentions) High performance liquid chromatography (hplc), by Tosoh (1 mentions) Radioimmunoassay kit, by Linco Research (1 mentions) 5890 gas chromatograph, by Hewlett-Packard (1 mentions) Adiponectin, by Thermo Fisher Scientific (1 mentions) Beckman glucose analyzer 2, by Beckman Coulter (1 mentions) Insulin, by Roche (1 mentions) Radioimmunoassay, by Linco Research (1 mentions) Infinity triglyceride kit, by Merck Group (1 mentions) Tnf α, by R&D Systems (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions)

Close spelling variants of this product

Human Adiponectin RIA Kit (7 citations) Human adiponectin ELISA kit (4 citations) Mouse Adiponectin ELISA Kit (3 citations) Quantikine Human Total Adiponectin Immunoassay (2 citations) Human HMW adiponectin ELISA kit (2 citations)

12 protocols using adiponectin

Plasma Adipokine Profiling in Mice

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Plasma leptin and adiponectin concentrations were analyzed by specific RIA for murine leptin (Linco Research) and adiponectin (Linco Research). Insulin was determined by means of a specific EIA kit for mouse insulin (Mercodia).

Gil-Ortega M., Martín-Ramos M., Arribas S.M., González M.C., Aránguez I., Ruiz-Gayo M., Somoza B, & Fernández-Alfonso M.S. (2016). Arterial stiffness is associated with adipokine dysregulation in non-hypertensive obese mice. Vascular pharmacology, 77.

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Measuring Adiponectin and Leptin Levels

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Fasting plasma adiponectin (Linco Research, St. Charles Missouri, USA) and leptin (Diagnostics Systems Laboratories, Webster, TX, USA) were measured using commercially available enzyme-linked immunoassay (ELISA) kits. Leptin: adiponectin ratio was calculated. The inter- and intra-assay coefficients of variation on pooled plasma specimens were as previously reported [29 (link)].

Al Mutairi S., Mojiminiyi O.A., Al Alawi A., Al Rammah T, & Abdella N. (2014). Study of Leptin and Adiponectin as Disease Markers in Subjects with Obstructive Sleep Apnea. Disease Markers, 2014, 706314.

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Anthropometric and Biochemical Examination

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A complete anthropometrical examination and a biochemical analysis were carried out on each patient. Height and weight were measured with the patient standing in light clothes and shoeless. BMI was calculated as body weight divided by height squared (kg/m²). Laboratory studies included glucose, insulin, glycated haemoglobin, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides, and transaminases, all of which were analysed using a conventional automated analyser. Insulin resistance (IR) was estimated using homeostasis model assessment of IR (HOMA2-IR) [23 (link)]. Serum levels of adiponectin (Linco Research, Inc., St. Charles, USA), resistin (Biovendor, Modrice, Czech Republic), interleukin 6 (IL6) (Quantikine, R&D Systems, Minneapolis, USA), tumour necrosis factor receptor 2 (TNFRII) (AssayPro, St. Charles, USA), and C-reactive protein (CRP) (Dade Behring, Marburg, Germany) were measured in duplicate using ELISA, following the manufacturer's instructions.

Auguet T., Berlanga A., Guiu-Jurado E., Terra X., Martinez S., Aguilar C., Filiu E., Alibalic A., Sabench F., Hernández M., Del Castillo D, & Richart C. (2014). Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease. BioMed Research International, 2014, 502542.

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Biomarker Measurement in Metabolic Disorders

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Serum AHSG concentration by radial immunodiffusion (RID) was as previous described elsewhere [36 (link)]. Intra-assay (IACV) and interassay IECV) coefficients of variation were below 5%. Adiponectin levels were measured with radioimmunoassay (IACV: 3.86%, IECV: 8.47%; Linco Research, St Charles, MO, USA). Serum tumor necrosis factor-α (TNFα, Sigma, St Louis, MO, USA; IACV: 4.8%, IECV: 6.7%), resistin (Linco Research Inc., IACV: 4.0%, IECV: 7.0%), and leptin (DRG International, Mountainside, NJ, USA, IACV%: 4.6%, IECV%: 6.6%) were measured by ELISA.
Fasting levels of serum glucose, total cholesterol, low-density (LDL) cholesterol, high-density (HDL) cholesterol, and triglycerides were detected by routine chemical laboratory methods. Insulin concentration was measured by insulin direct human ELISA kit (Invitrogen, Camarillo, CA, USA, lowest detectable concentration 0.17 μIU/ml, IACV: 4.8%, IECV: 81%). C-peptide was measured by RIA (Biodata, Rome, Italy, lowest detectable concentration: 0.2 ng/ml, IACV: 5.6%, IECV: 7.3%).

Temesszentandrási G., Vörös K., Márkus B., Böröcz Z., Kaszás E., Prohászka Z., Falus A., Cseh K, & Kalabay L. (2016). Human Fetuin-A Rs4918 Polymorphism and its Association with Obesity in Healthy Persons and in Patients with Myocardial Infarction in Two Hungarian Cohorts. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 2742-2750.

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Plasma Biomarker Profiling in Mice

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Plasma measurements were made on
a subpopulation of the mice (n = 6–8/diet).
Leptin and insulin concentrations were analyzed by ELISA kits (Leptin:
BioVendor, Czech Republic; Insulin: DRG Diagnostics, Germany). Glucagon
and Adiponectin we measured by radioimmunoassay kits (Glucagon: Millipore,
USA; Adiponectin: LINCO Research, USA). Plasma β-hydroxybutyrate
(OH-butyrate), triacylglycerol (TAG), free fatty acids (FFA), glucose,
and lactate concentrations were analyzed by conventional enzymatic
kits (β-hydroxybutyrate: Randox, United Kingdom; TG, FFA, and
glucose: DIALAB, Austria; lactate: Sentinel Diagnostics, Italy) using
a MaxMat PL II (MAXMAT S.A., Montpellier, France).

Lillefosse H.H., Clausen M.R., Yde C.C., Ditlev D.B., Zhang X., Du Z.Y., Bertram H.C., Madsen L., Kristiansen K, & Liaset B. (2014). Urinary Loss of Tricarboxylic Acid Cycle Intermediates As Revealed by Metabolomics Studies: An Underlying Mechanism to Reduce Lipid Accretion by Whey Protein Ingestion?. Journal of Proteome Research, 13(5), 2560-2570.

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Measurement of Plasma Metabolic Markers

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Plasma glucose was measured by the glucose oxidase method using a glucose analyzer (Yellow Springs Instrument Co., Yellow Springs, Ohio), insulin by commercially available radioimmunoassay (Millipore), HbA1c by high-performance liquid chromatography (Tosoh Medics) and adiponectin (ug/mL) by a radioimmunoassay kit (Linco Research) as previously reported (29 (link),37 (link)). The intra- and inter- assay coefficients for adiponectin were 3.6 and 9.3% for low, and 1.8 and 9.3% respectively for high serum concentrations (37 (link)). Plasma lipid concentrations were determined using the standards of the Centers for Disease Control and Prevention (13 (link)). Deuterium enrichment of glucose in the plasma was determined on a Hewlett-Packard Co. 5971 mass spectrometer (Palo Alto, CA) coupled to a 5890 gas chromatograph (13 (link)). Deuterium enrichment of glycerol in the plasma was determined according to previously described methods (24 (link)).

Gebara N.Y., Kim J.Y., Bacha F., Lee S, & Arslanian S. (2021). Metabolic Inflexibility in Youth with Obesity: Is it a Feature of Obesity or Distinctive of Youth Who are Metabolically Unhealthy?. Clinical obesity, 12(2), e12501.

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Plasma Insulin, Leptin, and Adiponectin Analysis

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The collected plasma was used for insulin, leptin and adiponectin concentration analysis using mouse insulin (Linco Research Inc., St. Charles, MO, USA), leptin (IDS Inc., Fountain Hills, AZ, USA) and adiponectin (Invitrogen, Grand Island, NY, USA) ELISA kits following manufacturer’s protocols.
The overall insulin sensitivity was estimated using the Homeostatic Model of Assessment (HOMA), representing relation between insulin and glucose concentrations, calculated by the formula (insulin×glucose)/22.5 (Matthews, Hosker et al. 1985 (link)).

Bennis M.T., Schneider A., Victoria B., Do A., Wiesenborn D.S., Spinel L., Gesing A., Kopchick J.J., Siddiqi S.A, & Masternak M.M. (2017). The role of transplanted visceral fat from the long-lived growth hormone receptor knockout mice on insulin signaling. GeroScience, 39(1), 51-59.

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Metabolic Measurements in Insulin Resistance

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Plasma was immediately separated from blood by centrifugation at 4°C for 10 min at 3600 rpm and stored at −20°C. Commercial radioimmunoassy kits were used to measure plasma insulin, leptin, and adiponectin (LINCO Research, St Charles, MO). During the clamp, plasma glucose concentrations were measured by the glucose oxidase method with a Beckman glucose analyzer II (Beckman Instruments, Fullerton, CA). Plasma 6,6-[²H₂]glucose isotopic enrichment was measured by gas chromatography-mass spectrometry (Hewlett-Packard Instruments), as previously described [21] (link). Breath ¹³CO₂ isotopic enrichment was determined by Isotope Ratio Mass Spectrometry (IR/MS) on a Tracermass C/N (SerCon Ltd, Crewe, Cheshire, UK). The homeostatic model assessment of insulin resistance index (HOMA-IR) and the HOMA corrected by adiponectin index (HOMA-AD) were calculated according to published formulas [7] (link).

Zanchi A., Tappy L., Lê K.A., Bortolotti M., Theumann N., Halabi G., Gauthier T., Mathieu C., Tremblay S., Bertrand P.C., Burnier M, & Teta D. (2014). Pioglitazone Improves Fat Distribution, the Adipokine Profile and Hepatic Insulin Sensitivity in Non-Diabetic End-Stage Renal Disease Subjects on Maintenance Dialysis: A Randomized Cross-Over Pilot Study. PLoS ONE, 9(10), e109134.

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Fasting Serum Adiponectin and Resistin Levels

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Blood samples were obtained after an overnight fast and stored at −80°C until analysis. Serum levels of adiponectin (Linco Research) and resistin (Linco Research) were measured by ELISA kits according to instructions. The intra- and inter-assay coefficients of variation were < 5% and < 10%, respectively.

Li R., Chen L.Z., Zhao S.P, & Huang X.S. (2016). Inflammation Activation Contributes to Adipokine Imbalance in Patients with Acute Coronary Syndrome. PLoS ONE, 11(3), e0151916.

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Quantifying Cord Blood Hormones

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We quantified the following hormones in cord blood hormones using commercial assays: leptin and adiponectin (radioimmunoassay; Linco Research Inc., St. Charles, MO)(23 (link)); insulin (competitive electrochemiluminescence immunoassay; Roche Diagnostics, Indianapolis, IN); insulin-like growth factor (IGF)-1 and IGF binding protein (IGFBP)-3 (ELISA, R&D Systems, Minneapolis, MN); and IGF-2 (ELISA, Alpco Diagnostics, Salem, NH). Day-to-day variabilities for each of these assays were <10%.

Perng W., Rifas-Shiman S.L., McCulloch S., Chatzi L., Mantzoros C., Hivert M.F, & Oken E. (2017). Associations of cord blood metabolites with perinatal characteristics, newborn anthropometry, and cord blood hormones in Project Viva. Metabolism: clinical and experimental, 76, 11-22.

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