Antibodies and dilutions used were as follows: ERα (ab32063: immunohistochemistry, 1:150; immunoblotting, 1:750; immunofluorescence, 1:150) from Abcam; vinculin (ab18058: immunohistochemistry, 1:100; immunoblotting, 1:400; immunofluorescence, 1:150) from Abcam; CD68 (916104: immunohistochemistry, 1:100) from BioLegend; GAPDH (CW0101: immunoblotting, 1:1,000) from CWBIOTECH; p-MLC(3671: immunoblotting, 1:750; immunofluorescence, 1:50) from Cell Signaling Technology; MLC (10906-1-AP, immunoblotting, 1:500) from Proteintech; F-actin (40734ES75: immunofluorescence, 1:100) from YEASEN; fluorescein isothiocyanate (FITC)-conjugated anti-rabbit and Cy3-conjugated anti-mouse antibodies from ZHUANGZHIBIO.
The inhibitors are as follows: in vivo: GM6001 (MedChem Express, Monmouth Junction, NJ, USA) was subcutaneously injected every 3 days at 100 mg per kg body weight57 (link). In vitro: GM6001 (27 nm).
The inhibitors are as follows: in vivo: GM6001 (MedChem Express, Monmouth Junction, NJ, USA) was subcutaneously injected every 3 days at 100 mg per kg body weight57 (link). In vitro: GM6001 (27 nm).