Mmp 11

EGF and its inhibitors (LY294002 and U0126) were obtained from Sigma-Aldrich Corp (St. Louis, MO). Quinazoline (QNZ) was purchased from Enzo Life International Inc. (Plymouth Meeting, PA). EGFR, STAT3, STAT5α, STAT5β, p65, and scramble small interfering (si) RNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Complementary DNA (cDNA) plasmids containing MMP-9 were purchased from Origene Technologies (Rockville, MD) and were subcloned to the pCMV6-XL vector. Antibodies against β-actin, MMP-9, MMP-11, EGF, EGFR, phosphorylated (p)-ERK1/2, ERK1/2, p-AKT, AKT, p-STAT3, STAT3, p-STAT5, and STAT5 were purchased from Cell Signaling Technology, Inc. (Danvers, MA). All reagents for quantitative reverse transcription polymerase chain reaction (qRT-PCR) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio-Rad Laboratories (Hercules, CA, USA). All other reagents were obtained from Invitrogen (Carlsbad, CA), unless otherwise specified.

The recombinant human IL-1β, PGE₂, forskolin, PGD₂, 15d-PGJ₂ and the inhibitors including NS398, LY294002, SB203580 and SP600125 were obtained from Sigma-Aldrich Corp. Quinazoline (QNZ) was obtained from Enzo Life Sciences (Farmingdale, NY, USA). Rofecoxib was purchased from Tangchao Chemical Inc. (Xian, SX, China). HO-1 siRNA was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies specific for β-actin, COX-2, Akt, p-Akt (Ser 473), p38, p-p38 (Thr 180/Tyr 182), c-Jun, p-c-Jun (Ser 63), NF-κB, p-NF-κB (Ser 536), p-NF-κB (Ser 276), IL-1β, FGF-2, MMP-1, MMP-11 and HO-1 were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Recombinant human FGF-2 was obtained from Raybiotech, Inc. (Norcross, GA, USA). The PGE₂ and IL-1β enzyme immunoassay kits were from Cayman Chemical (Ann Arbor, MI). 15d-PGJ₂ enzyme immunoassay kits were obtained from Assay Designs (Ann Arbor, MI, USA). FGF-2 enzyme immunoassay kits were purchased from Raybiotech, Inc. (Norcross, GA, USA). All reagents for qRT-PCR and SDS-PAGE experiments were purchased from Bio-Rad Laboratories. All other reagents were from Invitrogen (Carlsbad, CA), unless otherwise specified.