Anti β actin sc 5286

Mouse lung tissue samples were lysed in RIPA buffer (50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% Glycerol), and protease/phosphatase inhibitors, 0.3 mM dithiothreitol, 5 mM nicotinamide and 1 mM sodium butyrate were added to the buffer. Western blots were performed according to standard protocols. Antibodies and the dilutions were anti-GAPDH (10494-1-AP, Proteintech, 1:10000), anti-β-actin (sc-5286, Santa Cruz, 1:10000), anti-Syk (13198, Cell Signaling Technology, 1:1000), anti-Phospho-Syk (14140, Cell Signaling Technology, 1:1000), anti-EGFR (PA1-1110, Invitrogen, 1:1000), anti-Phospho-EGFR (8808, Cell Signaling Technology, 1:1000).

Mouse lung tissue samples were lysed in RIPA buffer (50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 10% glycerol) with protease/phosphatase inhibitor cocktail (5872S, Cell Signaling Technology, 1:1000), 0.3 mM DTT, 5 mM nicotinamide, and 1 mM sodium butyrate. Total 500 μg of protein were used for immunoprecipitation with indicated dilution of each antibody (anti-STAT1, 14994, Cell Signaling Technology, 1:100).
Western blotting was performed according to standard protocols. The antibodies and dilutions were anti-β-actin (sc-5286, Santa Cruz, 1:10,000), anti-STAT1 (14994, Cell Signaling Technology, 1:1000), anti-acetyl-lysine (PTM-105, PTM Bio, 1:1000), and anti-succinyl-lysine (PTM-401, PTM Bio, 1:1000).