Gv280

Manufactured by Genechem

Sourced in China

The GV280 is a laboratory instrument designed for conducting centrifugation processes. It features a compact design and can accommodate various rotor types to suit different sample volumes and applications. The GV280 provides consistent and reliable performance for a range of scientific research and diagnostic tasks.

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Lab products found in correlation

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GV280 lentiviral vector (4 citations) GV280 vector (3 citations) GV369/GV280 vector (2 citations)

4 protocols using gv280

Modulating EBV-miR-BART8-3p Expression in Nasopharyngeal Carcinoma Cells

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Lentiviral particles (GV369 and Ubi-MCS-SV40-EGFP-IRES-puromycin) containing EBV-miR-BART8-3p precursors and lentiviral particles (GV280 and hU6-MCS-Ubiquitin-EGFP-IRES-puromycin) containing reverse complement of EBV-miR-BART8-3p and their control vectors were constructed by Shanghai Genechem Co., Ltd. (Shanghai, China).CNE-1 and SUNE-1 cells were transfected with a recombinant lentiviral vector GV369 to upregulate EBV-miR-BART8-3p expression (CNE-1-BART8-3p and SUNE-1-BART8-3p cells), and C666–1 cells were transfected with a lentiviral vector GV280 to downregulate EBV-miR-BART8-3p expression (C666–1-BART8-3p cells). The transfection efficiency was checked using qPCR assay.
For the rescue assay, CNE-1-BART8-3p cells and SUNE-1-BART8-3p cells were transfected with the RNF38 lentiviral vector GV358 (Shanghai Genechem Co., Ltd.; Shanghai, China) or a normal control (Shanghai Genechem Co., Ltd.; Shanghai, China).

Lin C., Zong J., Lin W., Wang M., Xu Y., Zhou R., Lin S., Guo Q., Chen H., Ye Y., Zhang B, & Pan J. (2018). EBV-miR-BART8-3p induces epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma cells through activating NF-κB and Erk1/2 pathways. Journal of Experimental & Clinical Cancer Research : CR, 37, 283.

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Generating Stable Cell Lines for PLPP4 Knockdown

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Short hairpin (shRNA) RNA for human PLPP4 was cloned into a phU6-MCS-Ubiquitin -EGFP-IRES-puromycin plasmid (GV280, Genechem, China), and the list of primers for the clone reactions is presented in Additional file 5: Table S5. Transfection of the plasmids was performed using Lipofectamine 3000 reagent (Invitrogen, USA) according to the manufacturer’s instructions. Stable cell lines expressing shPLPP4#1or shPLPP4#2 were generated by lentiviral infection using HEK293T cells, and selected with 0.5 mg/L puromycin for 10 days. The luciferase reporter system of pE2F-luc, pRb-luc and pGL3-luc (Clontech) was used to examine the transcriptional activity of E2F and Rb.

Zhang X., Zhang L., Lin B., Chai X., Li R., Liao Y., Deng X., Liu Q., Yang W., Cai Y., Zhou W., Lin Z., Huang W., Zhong M., Lei F., Wu J., Yu S., Li X., Li S., Li Y., Zeng J., Long W., Ren D, & Huang Y. (2017). Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells. Molecular Cancer, 16, 147.

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Lentiviral Transduction of miR-874-3p and HDAC1

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miR-874-3p mimic non-targeting negative control (NC; 5'-ACUACUGAGUGACAGUAGA-3') or miR-874-3p mimic (5'-CUGCCCUGGCCCGAGGGACCGA-3') were inserted into the GV309 vector (Shanghai GeneChem Co., Ltd.); miR-874-3p inhibitor non-targeting NC (5'-CAGUACUUUUGUGUAGUACAA-3') or miR-874-3p inhibitor (5'-UCGGUCCCUCGGGCCAGGGCAG-3') were inserted into the GV280 vector (Shanghai GeneChem Co., Ltd.). For HDAC1 overexpression, the coding sequence of HDAC1 was inserted into the GV358 vector (Shanghai GeneChem Co., Ltd.). The empty vector was used as the NC for HDAC1 overexpression. 293T cells (American Type Culture Collection) were transfected with 20 µg the GV vector (GV309, GV280 or GV358) together with 15 µg pHelper 1.0 and 10 µg pHelper 2.0 vectors (both from Shanghai GeneChem Co., Ltd.) and then cultured at 37˚C in a humidified incubator in an atmosphere of 5% CO₂ for 48 h to produce lentiviral particles. Transduction was performed when cells were growing exponentially and were 70-80% confluent. Polybrene (8 µg/ml, Sigma-Aldrich; Merck KGaA) and appropriate lentiviral particles (10⁸ TU/ml, 5 µl) were added to cells for 16 h at 37˚C in a humidified incubator in an atmosphere of 5% CO₂. The medium containing lentiviral particles were removed from wells and fresh medium were added. Subsequent experiments were performed 24 h later.

Wei Y., Wang Z., Wei L., Li S., Qiu X, & Liu C. (2021). MicroRNA-874-3p promotes testosterone-induced granulosa cell apoptosis by suppressing HDAC1-mediated p53 deacetylation. Experimental and Therapeutic Medicine, 21(4), 359.

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Lentiviral Transduction of AP-2α, miR-193a-5p

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All the lentiviruses expressing AP-2α gene, AP-2α shRNA (target sequence: 5′-TTTCTCAACCGACAACATT-3′), has-miR-193a-5p precursor and has-miR-193a-5p inhibitor and their empty vector lentivirus (GV341, GV248, GV309, GV280, respectively) were purchased from GeneChem (Shanghai, China). Lentiviruses were transduced into cells at a multiplicity of infection (MOI) of 10 in the presence of polybrene.

Zhou J., Duan H., Xie Y., Ning Y., Zhang X., Hui N., Wang C., Zhang J, & Zhou J. (2016). MiR-193a-5p Targets the Coding Region of AP-2α mRNA and Induces Cisplatin Resistance in Bladder Cancers. Journal of Cancer, 7(12), 1740-1746.

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