After treatments, cells were lysed and Western blot was conducted as previously described55 (link). Proteins were visualized using primary antibodies recognizing HIF1α (BD Biosciences, #610959, San Jose, CA), HIF2α (Novus Biological, #NB100–122, Littleton, CO), α-tubulin (Fitzgerald, #10R-842, North Acton, MA), MYPT1-P Thr696 (EMD Millipore, #ABS45, Temecula, CA), MYPT1, (Abcam, #ab32393, Cambridge, MA), Cleaved Caspase 3, VHL (Cell Signaling, #9661, #2738, Danvers, MA), ROCK1, ROCK2 (Thermo Scientific, #PA5–22262, #PA5–21131, Grand Island, NY); and Horseradish peroxidase conjugated Goat anti-Rabbit IgG and Goat anti-Mouse IgG secondary antibodies (Thermo Scientific, #31460, #31430). Blots were imaged using a Bio Rad ChemiDoc XRS+ (BioRad, Hercules, CA).
Horseradish peroxidase conjugated goat anti rabbit igg and goat anti mouse igg secondary antibodies
Manufactured by Thermo Fisher Scientific
Horseradish peroxidase (HRP) conjugated Goat anti-Rabbit IgG and Goat anti-Mouse IgG secondary antibodies are proteins that bind to and detect primary antibodies raised in rabbits or mice. The HRP enzyme provides a signal for visualization or quantification in immunoassays and other applications.
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2 protocols using horseradish peroxidase conjugated goat anti rabbit igg and goat anti mouse igg secondary antibodies
1
Western Blot Analysis of Cellular Signaling
2
Western Blot Analysis of Cellular Signaling
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After treatments, cells were lysed and Western blot was conducted as previously described55 (link). Proteins were visualized using primary antibodies recognizing HIF1α (BD Biosciences, #610959, San Jose, CA), HIF2α (Novus Biological, #NB100–122, Littleton, CO), α-tubulin (Fitzgerald, #10R-842, North Acton, MA), MYPT1-P Thr696 (EMD Millipore, #ABS45, Temecula, CA), MYPT1, (Abcam, #ab32393, Cambridge, MA), Cleaved Caspase 3, VHL (Cell Signaling, #9661, #2738, Danvers, MA), ROCK1, ROCK2 (Thermo Scientific, #PA5–22262, #PA5–21131, Grand Island, NY); and Horseradish peroxidase conjugated Goat anti-Rabbit IgG and Goat anti-Mouse IgG secondary antibodies (Thermo Scientific, #31460, #31430). Blots were imaged using a Bio Rad ChemiDoc XRS+ (BioRad, Hercules, CA).
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