Total protein were lysed by using radioimmunoprecipitation assay (RIPA) buffer (Invitrogen). Equal amount of proteins were resolved using denaturing SDS–PAGE and analyzed by WB using antibodies recognizing β-catenin and β-actin (Santa Cruz Biotechnology Inc, US).
Antibodies recognizing β catenin and β actin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibodies recognizing β-catenin and β-actin are laboratory reagents used for the detection and analysis of these proteins in biological samples. The anti-β-catenin antibody specifically binds to the β-catenin protein, while the anti-β-actin antibody recognizes the β-actin protein. These antibodies can be used in various techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of these proteins in cells and tissues.
Automatically generated - may contain errors
2 protocols using antibodies recognizing β catenin and β actin
1
Protein Extraction and Western Blot
2
Western Blot Analysis of β-Catenin
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Drug treated cells were washed with ice cold PBS on ice and lysed in RIPA lysis buffer (Life Technologies) supplemented with protease inhibitor cocktail (Roche). Protein concentration were determined using BCA protein assay kit (Pierce). 20-40 µg of protein lysate per sample was resolved on SDS-polyacrylamide gels and transferred onto PVDF membrane (Millipore). Western blotting was performed using antibodies recognizing β-catenin and β-actin (Santa Cruz Biotechnology). Signals were detected on Li-COR Odyssey imaging system.
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