Anti mouse igg dylight 649

Phorbol-12-myristate-13-acetate (PMA), gelatin, Triton X-100, Coomassie brilliant blue R-250 was purchased from Sigma-Aldrich, MMP-9 inhibitor (SB-3CT) and NF-κB inhibitor (sc-75741) were purchased from Selleck. Recombinant human MMP-9 protein and Recombinant mice MMP-9 protein were purchased from R&D systems. Recombinant human pro-MMP-9 protein were purchased from ProSpec. Commercialized DENV2-NS1 protein were purchased from Native Antigen. Trizol reagent was purchased from Ambion. Lipofectamine 2000 reagent was purchased from Invitrogen. Human MMP-9 ELISA kit was purchased from BD Biosciences. Membrane maker Dil (D8700) was purchased from Solarbio. NF-κB Pathway Sampler Kit (#9936T), Tight Junction Antibody Sampler Kit (#8683T), Cadherin-Catenin Antibody Sampler Kit (#9961T), and Antibodies against TIMP-1 (8946S) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) were purchased from Genetex. Antibodies against DENV2-NS1 were purchased from Arigo biolaboratories (SQab1501). Antibodies against Flag (F3165) and HA (H6908) were purchased from Sigma. Anti-β-actin antibody (66009) were purchased from Proteintech. Rabbit IgG and Mouse IgG were purchased from Invitrogen. Anti-Mouse IgG Dylight 649, Anti-Mouse IgG Dylight 488, Anti-Rabbit IgG Dylight 649, and Anti-Rabbit IgG FITC were purchased from Abbkine.

Phorbol-12-myristate-13-acetate (PMA), LPS, nigericin, ATP, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich. Caspase-1 inhibitor (VX-765) was purchased from Selleck. Recombinant human IL-1β protein (200-01B) and human IL-1RA (200-01RA) were obtained from Peprotech. Recombinant mouse IL-1β protein (401-ML/CF) and recombinant mouse IL-1RA (480-RM/CF) were purchased from R&D Systems. Trizol reagent was from Invitrogen, and Lipofectamine 2000 was from Invitrogen. The human IL-1β ELISA kit was purchased from BD Biosciences, the mouse IL-1β and tumor necrosis factor (TNF)-α ELISA kits were purchased from R&D Systems, and the LPS ELISA kit was purchased from Expandbio (Beijing, China).
Antibodies against NLRP3 (D4d8T), caspase-1 (D7F10), and IL-1β (D3U3E and 3A6) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) and DENV-Prm (GTX128092) were purchased from Genetex. Anti-β-actin antibody (66009) was purchased from Proteintech. Antibody against ASC (sc-271054) was purchased from Santa Cruz Biotechnology. Anti-mouse IgG Dylight 649, anti-mouse IgG Dylight 488, anti-rabbit IgG Dylight 649, and anti-rabbit IgG fluorescein isothiocyanate (FITC) were purchased from Abbkine.

Anti-IRAK1 (D51G7) (Cat#4504), Anti-TRAF6 (D21G3) (Cat#8028), anti-Myc (9B11) (Cat#2276), anti-ubiquitin (P4D1) (Cat#3936), anti-K48-linkage-specific polyubiquitin (D9D5) (Cat#8081), anti-K63-linkage-specific polyubiquitin (D7A11) (Cat#5621), anti-p65 (Cat#8242), and anti-phospho-p65 (Ser536) (Cat#3033) were purchased from Cell Signaling Technology (Beverly, MA, USA).Anti-TRAF6 (Cat#ab137452) was purchased from Abcam (Cambridge, MA, USA). Anti-Flag (Cat# F3165) and anti-HA (Cat# H6908) antibodies were purchased from Sigma-Aldrich. Anti-TRAF6 (Cat# 66498-1-lg) and anti-GAPDH (Cat# 60004-1-lg) were purchased from Proteintech (Wuhan, Hubei, China). Anti-Rabbit IgG FITC (Cat# A22120) and anti-Mouse IgG DyLight 649 (Cat# A23610) antibodies were purchased from Abbkine.