Em scd005 cool sputter coater 7001f

Manufactured by Leica

Sourced in Germany

The Leica EM-SCD005 cool sputter coater 7001F is a device designed for the deposition of conductive coatings on samples for electron microscopy. It operates by sputtering a thin layer of material, such as gold or carbon, onto the surface of the sample to enhance its conductivity and improve image quality during scanning electron microscope (SEM) observation.

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Lab products found in correlation

Mira3 feg sem, by TESCAN (3 mentions)

Spelling variants of this product

EM SCD005 (32 citations) Sputter coater (23 citations) EM SCD005 sputter coater (8 citations) Leica sputter (4 citations) SCD005 sputter coater (2 citations)

3 protocols using em scd005 cool sputter coater 7001f

Scaffold Preparation for SEM Imaging

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Scaffolds were fixed
for 3 h with 2.5% w/v glutaraldehyde and then washed three times with
PBS. The samples were then dehydrated via a series of ethanol treatments
with increasing concentrations from 10% to ∼100% v/v by using
a Pelco BioWave Microwave Tissue processor. Once dehydrated, the scaffolds
were coated with a 5 nm layer of platinum via a Leica EM-SCD005 cool
sputter coater 7001F (Leica Microsystems GmbH, Germany). Bacteria
adhered to coated and uncoated scaffolds were then imaged using SEM
(Tescan MIRA3 FEG-SEM, Australia) at a voltage of 3 kV (spot size
11.86).

Cometta S., Jones R.T., Juárez-Saldivar A., Donose B.C., Yasir M., Bock N., Dargaville T.R., Bertling K., Brünig M., Rakić A.D., Willcox M, & Hutmacher D.W. (2022). Melimine-Modified 3D-Printed Polycaprolactone Scaffolds for the Prevention of Biofilm-Related Biomaterial Infections. ACS Nano, 16(10), 16497-16512.

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Scaffold Microstructure Analysis by SEM

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Changes on the morphological microstructure of treated and untreated scaffolds were analyzed by SEM (Tescan MIRA3 FEG-SEM, Australia) at a voltage of 10 kV and a spot size of 12.12. Scaffolds were gold-coated for 75 s at 30 mA by using a Leica EM-SCD005 cool sputter coater 7001F (Leica Microsystems GmbH, Germany).

Cometta S., Bock N., Suresh S., Dargaville T.R, & Hutmacher D.W. (2021). Antibacterial Albumin-Tannic Acid Coatings for Scaffold-Guided Breast Reconstruction. Frontiers in Bioengineering and Biotechnology, 9, 638577.

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Scanning Electron Microscopy of S. aureus Adherence

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Staphylococcus aureus (ATCC 29213) colonies were resuspended in MH broth at a concentration of 1 × 10⁸ CFU/mL and placed into 48-well plates (1 mL/well). Treated and untreated scaffolds were placed in each well and incubated at 37°C overnight. Scaffolds with adherent S. aureus were washed twice with PBS and subsequently fixed for 3 h with 2.5% glutaraldehyde. Samples were then dehydrated via a series of ethanol treatment by using a Pelco BioWave Microwave Tissue processor. Once dehydrated, the scaffolds were platinum-coated for 75 s at 30 mA by using a Leica EM-SCD005 cool sputter coater 7001F (Leica Microsystems GmbH, Germany). Adhered bacteria to the coated and uncoated scaffolds were then examined under SEM (Tescan MIRA3 FEG-SEM, Australia) at a voltage of 5 kV (spot size 12.12).

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