Angiogenesis assay was conducted using 6-well plates that were pre-coated with Matrigel (BD Biosciences) at 37°C for 24 h. Subsequently, 1×105 HUVECs were seeded into each well. Following 24 h cell culture at 37°C, the formation of capillary-like structures was photographed and the branchpoints were counted. The tube length was analyzed by the AxioVision Rel software version 4.8 (Carl Zeiss AG).
Axiovision rel software version 4
Manufactured by Zeiss
Sourced in Germany
AxioVision Rel software version 4.8 is a comprehensive imaging and analysis software solution developed by Zeiss. It provides a platform for acquiring, processing, analyzing, and managing digital images from a variety of microscopy systems. The software offers a range of tools and functionalities to support researchers and scientists in their imaging workflows.
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Lab products found in correlation
3 protocols using axiovision rel software version 4
1
Angiogenesis Assay of HUVECs on Matrigel
2
Angiogenesis Assay with HUVECs
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For angiogenesis assay, 6-well plates were pre-coated with matrigel (BD Biosciences, San Jose, CA, USA). Then, 1×105 HUVECs cells were seeded into each well. After 24 h of culturing at 37°C, the formation of capillary-like structures was photographed and the branch points were counted. The tube length was analyzed by the AxioVision Rel software version 4.8 (Carl Zeiss AG, Jena, Germany).
3
Cartilage Degeneration Quantification
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Femoral and tibial condyles were dissected separately without damaging the cartilage surface, and then stained with India ink to assess cartilage degeneration. Quantification of macroscopic images was evaluated by the area of macroscopic cartilage degeneration. Macroscopic pictures were taken using a ZEISS Stimi 2000C microscope (Carl Zeiss, Oberkochen, Germany) on a dedicated medical photography platform. The degeneration area of the medial tibial plateau was measured using AxioVision Rel software version 4.8 (Carl Zeiss, Oberkochen, Germany). Macroscopic assessment was performed separately by two examiners and the average values were recorded.
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