Three mixes were utilized in this study: AZD4547 (Selleck Chem, S2801) was diluted to 1 µM in 4% dimethyl sulfoxide+30% polyethylene glycol 300+5% Tween 80+ddH2O; teriparatide acetate (Selleck Chem, P1033) was diluted to 1 µM in ddH2O; AZTeri was a mix of 1 µM AZD4547 and 1 µM teriparatide acetate diluted in ddH2O. Embryos were treated at HH33 via applying 10 µl of the drugs under the chorioallantoic membrane immediately adjacent to the mandible. Embryos were then incubated without shaking in the incubator. Wholemount heads were dissected at either HH34 or HH39 and processed for further analysis.
Teriparatide acetate
Manufactured by Selleck Chemicals
Teriparatide acetate is a synthetic parathyroid hormone used as a laboratory reagent. It is primarily utilized in research and scientific experimentation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using teriparatide acetate
1
Mandibular Development Modulation Protocol
2
Localized Mandibular Drug Delivery
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Three mixes were utilized in this study: AZD4547 (Selleck Chem -S2801) was diluted to 1µM in 4% DMSO+30% PEG 300+5% Tween 80+ddH2O. Teriparatide Acetate (Selleck Chem -P1033) was diluted to 1µM in ddH2O. AZTeri was a mix of 1µM of AZD4547 and 1µM of Teriparatide Acetate diluted in ddH2O.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Embryos were treated at HH33 embryos via applying 10µL of the drugs under the chorioallantoic membrane immediately adjacent to the mandible. Embryos were then incubated without shaking in the incubator. Wholemount heads were dissected at either HH34 or HH39 and processed for further analysis.
Analysis of serum calcium content 100µl of blood was collected from the vitelline vein of HH39 embryos was collected on ice with microcapillaries, weighted and sent for processing by the R. Marshall Wilson Mass Spectrometry facility at University of Cincinnati. Inductively Coupled Plasma -Mass Spectrometry with High Performance Liquid Chromatography (ICP-MS HPLC) was utilized.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Embryos were treated at HH33 embryos via applying 10µL of the drugs under the chorioallantoic membrane immediately adjacent to the mandible. Embryos were then incubated without shaking in the incubator. Wholemount heads were dissected at either HH34 or HH39 and processed for further analysis.
Analysis of serum calcium content 100µl of blood was collected from the vitelline vein of HH39 embryos was collected on ice with microcapillaries, weighted and sent for processing by the R. Marshall Wilson Mass Spectrometry facility at University of Cincinnati. Inductively Coupled Plasma -Mass Spectrometry with High Performance Liquid Chromatography (ICP-MS HPLC) was utilized.
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