Kmbc2

Seven BC cell lines (T24, RT112, 253 J‐BV, KMBC2, UMUC3, UMUC6, UMUC13) were grown in RPMI‐1640 (Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) containing 10% fetal bovine serum (BioWhittaker, Walkersville, MD). Cells were cultured in a humidified incubator at 37°C with 5% CO₂. T24 and KMBC2 were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), and the other cell lines were kindly provided by Prof. Peter C. Black (Department of Urologic Sciences, Vancouver Prostate Centre, University of British Columbia, Vancouver, Canada). Authentication of the cell lines was confirmed by short tandem repeat (STR) profiling.
The primer sequences for qRT‐PCR, RNA interference (RNAi), expression vector, proliferation assays, cell migration assays, invasion assays, spheroid colony formation assays, and statistical methods are presented in Data S1.

The human bladder cancer-derived cell lines UMUC7, UMUC14, RT112, RT4, VMCUB1, SCaBER, UMUC6, T24 and HT1197 were obtained from DSMZ (Heidelberg, Germany). KMBC2 cells were purchased from JCRB cell bank (Japan). KMBC2 cells were cultured at 37 °C in an atmosphere of 5% CO₂ in Ham F12 medium, RT112 and RT4 cells were cultured in RPMI medium and all the other cells were cultured in DMEM medium. All cell media were supplemented with 10% fetal bovine serum (FBS). Cells were routinely tested for mycoplasma contamination.