Genesig easy rna internal extraction control

Manufactured by Primerdesign

Sourced in United Kingdom

The GenesigⓇ Easy RNA Internal extraction control is a laboratory reagent designed to monitor the RNA extraction process. It is used to verify the efficiency of RNA extraction and to identify potential inhibition of the reverse transcription and/or amplification steps.

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Lab products found in correlation

Maxwell rsc 48 instrument, by Promega (2 mentions) Maxwell rsc viral total nucleic acid purification kit, by Promega (1 mentions)

2 protocols using genesig easy rna internal extraction control

Maxwell RSC Viral RNA Extraction

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RNA was extracted using the Maxwell^Ⓡ RSC Viral Total Nucleic Acid Purification Kit (Promega UK Ltd., Southampton, UK) according to manufacturer's instructions. Briefly, a Class 1 microbiological safety cabinet (MSC) within a containment level 3 laboratory, 200 µl of sample was added to 223 µl of prepared lysis solution (including 5 µl per reaction of Genesig^Ⓡ Easy RNA Internal extraction control, Primerdesign Ltd, Chandler's Ford, UK). Samples were then inactivated for 10 min at room temperature within the MSC and 10 min at 56 °C on a heat block before automated RNA extraction using a Maxwell^Ⓡ RSC 48 Instrument (Promega UK Ltd., Southampton, UK). RNA was eluted in 50 µl of NFW. In the case of saliva, RNA was extracted from 200 µl of saliva diluted 1:20, as saliva volume was insufficient unless a dilution was performed.

Fowler V.L., Armson B., Gonzales J.L., Wise E.L., Howson E.L., Vincent-Mistiaen Z., Fouch S., Maltby C.J., Grippon S., Munro S., Jones L., Holmes T., Tillyer C., Elwell J., Sowood A., de Peyer O., Dixon S., Hatcher T., Patrick H., Laxman S., Walsh C., Andreou M., Morant N., Clark D., Moore N., Houghton R., Cortes N.J, & Kidd S.P. (2020). A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection. The Journal of Infection, 82(1), 117-125.

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SARS-CoV-2 RNA Extraction Protocol

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In a class 1 microbiological safety cabinet within a containment level 3 laboratory, 200 μL of sample was added to 223 μL of prepared lysis solution (including 5 μL per reaction of Genesig Easy RNA Internal extraction control; Primerdesign Ltd, Chandler's Ford, UK). Samples were then inactivated for 10 minutes at room temperature within the microbiological safety cabinet and 10 minutes at 56°C on a heat block before automated RNA extraction using a Maxwell RSC48 Instrument (Promega UK Ltd, Southampton, UK). RNA was eluted in 50 μL of nuclease-free water.

Kidd S.P., Burns D., Armson B., Beggs A.D., Howson E.L., Williams A., Snell G., Wise E.L., Goring A., Vincent-Mistiaen Z., Grippon S., Sawyer J., Cassar C., Cross D., Lewis T., Reid S.M., Rivers S., James J., Skinner P., Banyard A., Davies K., Ptasinska A., Whalley C., Ferguson J., Bryer C., Poxon C., Bosworth A., Kidd M., Richter A., Burton J., Love H., Fouch S., Tillyer C., Sowood A., Patrick H., Moore N., Andreou M., Morant N., Houghton R., Parker J., Slater-Jefferies J., Brown I., Gretton C., Deans Z., Porter D., Cortes N.J., Douglas A., Hill S.L., Godfrey K.M, & Fowler V.L. (2022). Reverse-Transcription Loop-Mediated Isothermal Amplification Has High Accuracy for Detecting Severe Acute Respiratory Syndrome Coronavirus 2 in Saliva and Nasopharyngeal/Oropharyngeal Swabs from Asymptomatic and Symptomatic Individuals. The Journal of Molecular Diagnostics : JMD, 24(4), 320-336.

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