Ag 20b 0042

Manufactured by Enzo Life Sciences

The AG-20B-0042 is a laboratory instrument designed for measuring the absorbance of liquid samples. It utilizes a light source and a detector to measure the amount of light absorbed by the sample at a specific wavelength, providing quantitative data about the chemical composition or concentration of the sample.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (2 mentions) Pro caspase 8, by Enzo Life Sciences (2 mentions) Il 18, by Cell Signaling Technology (1 mentions) Ag 20b 0014, by R&D Systems (1 mentions) Caspase 11, by Novus Biologicals (1 mentions) Cleaved caspase 8, by Cell Signaling Technology (1 mentions) Il 1β, by R&D Systems (1 mentions) Ag 20b 0014, by Cell Signaling Technology (1 mentions)

2 protocols using ag 20b 0042

Immunoblotting Analysis of Apoptosis Markers

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Samples for immunoblotting were prepared by combining cell lysates with culture supernatants. Samples were denatured in loading buffer containing SDS and 100 mM DTT and boiled for 5 min. SDS-PAGE-separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against caspase-1 (Adipogen, AG-20B-0042), pro-caspase-8 (Enzo Life Sciences, 1G12), cleaved caspase-8 (Cell Signaling Technology, D5B2), caspase-11 (Novus Biologicals, 17D9), FADD (Millipore, 1F7), Nlrp3 (Adipogen, AG-20B-0014), IL-1β (R&D Systems), IL-18 (31 (link)) and GAPDH (Cell Signaling Technology, D16H11) followed by secondary anti-rabbit or anti-rat or anti- mouse or anti-goat HRP antibodies (Jackson Immuno Research Laboratories), as previously described(22 (link)).

Gurung P., Anand P.K., Subbarao Malireddi R.K., Walle L.V., Van Opdenbosch N., Dillon C.P., Weinlich R., Green D.R., Lamkanfi M, & Kanneganti T.D. (2014). FADD and caspase-8 mediate priming and activation of the canonical and non-canonical Nlrp3 inflammasomes. Journal of immunology (Baltimore, Md. : 1950), 192(4), 1835-1846.

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Immunoblotting of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples for immunoblotting were prepared by combining cell lysates with culture supernatants. Samples were denatured in loading buffer containing SDS and 100 mM DTT and boiled for 5 min. SDS-PAGE-separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against caspase-1 (Adipogen, AG-20B-0042), pro-caspase-8 (Enzo Life Sciences, 1G12), cleaved caspase-8 (Cell Signaling Technology, D5B2), NLRP3 (Adipogen, AG-20B-0014), IL-1β (Cell Signaling Technology, D3H1Z), and GAPDH (Cell Signaling Technology, D16H11) followed by secondary anti-rabbit or anti-rat or anti- mouse HRP antibodies (Jackson Immuno Research Laboratories), as previously described24 (link).

Gurung P., Li B., Subbarao Malireddi R.K., Lamkanfi M., Geiger T.L, & Kanneganti T.D. (2015). Chronic TLR Stimulation Controls NLRP3 Inflammasome Activation through IL-10 Mediated Regulation of NLRP3 Expression and Caspase-8 Activation. Scientific Reports, 5, 14488.

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