PECs were collected and washed once. For latency experiments, cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). Total PECs were used for MHV68 acute infection experiments. CD11b+ cells or whole PECs were Fc blocked with α-CD16/32 (Biolegend) and stained with Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend), APC-Cy7-α-CD19 (1D3, BD Biosciences), redFluor 710-α-CD11b (M1/70, Tonbo) or APC-Cy7-α-CD11b (M1/70, Biolegend), and R718-α-CD102 (3C4 (MIC2/4), BD Biosciences). Cells were counted again and resuspended in 3 x 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour, then washed with PBS and fixed with 2% formaldehyde. Samples were immediately run on a Novocyte 3000 (ACEA Biosciences).
Redfluor 710 α cd11b m1 70
Manufactured by BioLegend
RedFluor 710-α-CD11b (M1/70) is a fluorescent-conjugated antibody that binds to the CD11b molecule on the surface of cells. CD11b is an integrin that plays a role in cell adhesion and migration. This product can be used for the identification and analysis of cells expressing CD11b in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b microbead kit, by Miltenyi Biotec (2 mentions)
αcd16 32, by BioLegend (2 mentions)
Liveblazer fret b g loading kit with ccf4 am, by Thermo Fisher Scientific (2 mentions)
Alexafluor 647 α cd102 3c4 mic2 4, by BioLegend (2 mentions)
Apc cy7 α cd19 1d3, by BD (2 mentions)
Apc cy7 α cd11b m1 70, by BioLegend (2 mentions)
R718 α cd102 3c4 mic2 4, by BD (2 mentions)
Novocyte 3000, by Agilent Technologies (2 mentions)
Ls column, by Miltenyi Biotec (2 mentions)
2 protocols using redfluor 710 α cd11b m1 70
1
Purification and Characterization of CD11b+ Cells
2
Purification and Characterization of CD11b+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PECs were collected and washed once. For latency experiments, cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). Total PECs were used for MHV68 acute infection experiments. CD11b+ cells or whole PECs were Fc blocked with α-CD16/32 (Biolegend) and stained with Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend), APC-Cy7-α-CD19 (1D3, BD Biosciences), redFluor 710-α-CD11b (M1/70, Tonbo) or APC-Cy7-α-CD11b (M1/70, Biolegend), and R718-α-CD102 (3C4 (MIC2/4), BD Biosciences). Cells were counted again and resuspended in 3 x 106 cells/mL before adding CC4F-AM (LiveBLAzer FRET-B/G Loading Kit with CCF4-AM, ThermoFisher Scientific). Cells were incubated for 1 hour, then washed with PBS and fixed with 2% formaldehyde. Samples were immediately run on a Novocyte 3000 (ACEA Biosciences).
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