Mouse mcp 1 single plex

The following serum cytokine concentrations were quantified using multiplex and single immunoassays (Mouse Pro-inflammatory 7-plex and Mouse MCP-1 single-plex, Meso Scale Discovery, Rockville, MD): interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), chemokine C-X-C motif ligand 1 (CXCL1), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and monocyte chemotactic protein-1 (MCP-1). Measurements were performed on a SECTOR Imager 2400 (Meso Scale Discovery, Rockville, MD). The mean intra-assay CVs for the 7-plex pro-inflammatory and MCP-1 immunoassay were 3.3% and 2.8%, respectively. The lower limit of detection (LLOD) for each cytokine is as follows: IL-1β (0.21 pg/ml), IL-6 (3.07 pg/ml), IL-10 (0.37 pg/ml), IL-12p70 (9.73 pg/ml), CXCL1 (0.27 pg/ml), TNF-α (0.63 pg/ml), IFN-γ (0.06 pg/ml), and MCP-1 (1.46 pg/ml). One half of the LLOD was used for any value below the level of detection for the purpose of performing statistical analyses. Concentrations of serum TNF-α and IL-12p70 were below the LLOD in a majority of samples and so were not included in the analysis.

The serum cytokines interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), chemokine C-X-C motif ligand 1 (CXCL1), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), and monocyte chemotactic protein-1 (MCP-1) were quantified using multiplex and single immunoassays (Mouse Pro-inflammatory 7-plex and Mouse MCP-1 single-plex, Meso Scale Discovery, Rockville, MD) in triplicate. The mean intra-assay coefficients of variation for the 7-plex pro-inflammatory and MCP-1 immunoassay were 3.3% and 2.8%, respectively. A SECTOR Imager 2400 (Meso Scale Discovery, Rockville, MD) was used for all serum measurements.