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μ slide 4 0.4 flow chambers

Manufactured by Ibidi
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The μ-Slide IV 0.4 flow chambers are a type of lab equipment designed for microscopic observation and analysis of cell behavior under controlled flow conditions. They provide a defined fluidic environment for live-cell imaging applications.

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Lab products found in correlation

Dm16000b widefield microscope, by Leica (2 mentions)

Close spelling variants of this product

μ-Slide VI 0.4 flow chambers μ-slide chambers μ-Chamber slides Flow chamber slides μ-slide IV μ-slides Chamber slides Flow chambers

2 protocols using μ slide 4 0.4 flow chambers

1

Adhesion of Melanoma Cells to Endothelial Monolayers

Check if the same lab product or an alternative is used in the 5 most similar protocols
HLECs were grown to form a monolayer on gelatin-coated M6 plates and treated with 5 μg/ml of sEVs for 72 h. CSFE-labeled 50,000 SK-MEL-147 cells were then added to each well. Cells were gently washed twice with medium 3 h later and fixed in 4% PFA. Images were taken with a Leica LED microscope. For analysis in flow conditions, HMVECs plated in μ-Slide IV 0.4 flow chambers (Ibidi) were incubated during 24 h with 5 μg/ml PKH-67-labeled sEVs. Subsequently a flow of SK-MEL-147 cells (500,000 cells/mL) at 0.0625 mL/min passed through the flow chambers during 4 h. Images were taken on a DM16000B Widefield microscope (Leica Microsystems) and analyzed with FIJI 2.0 software.
García-Silva S., Benito-Martín A., Nogués L., Hernández-Barranco A., Mazariegos M.S., Santos V., Hergueta-Redondo M., Ximénez-Embún P., Kataru R.P., Lopez A.A., Merino C., Sánchez-Redondo S., Graña-Castro O., Matei I., Nicolás-Avila J.Á., Torres-Ruiz R., Rodríguez-Perales S., Martínez L., Pérez-Martínez M., Mata G., Szumera-Ciećkiewicz A., Kalinowska I., Saltari A., Martínez-Gómez J.M., Hogan S.A., Saragovi H.U., Ortega S., Garcia-Martin C., Boskovic J., Levesque M.P., Rutkowski P., Hidalgo A., Muñoz J., Megías D., Mehrara B.J., Lyden D, & Peinado H. (2021). Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism.. Nature cancer, 2(12), 1387-1405.
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2

Adhesion of Melanoma Cells to Endothelial Monolayers

Check if the same lab product or an alternative is used in the 5 most similar protocols
HLECs were grown to form a monolayer on gelatin-coated M6 plates and treated with 5 μg/ml of sEVs for 72 h. CSFE-labeled 50,000 SK-MEL-147 cells were then added to each well. Cells were gently washed twice with medium 3 h later and fixed in 4% PFA. Images were taken with a Leica LED microscope. For analysis in flow conditions, HMVECs plated in μ-Slide IV 0.4 flow chambers (Ibidi) were incubated during 24 h with 5 μg/ml PKH-67-labeled sEVs. Subsequently a flow of SK-MEL-147 cells (500,000 cells/mL) at 0.0625 mL/min passed through the flow chambers during 4 h. Images were taken on a DM16000B Widefield microscope (Leica Microsystems) and analyzed with FIJI 2.0 software.
García-Silva S., Benito-Martín A., Nogués L., Hernández-Barranco A., Mazariegos M.S., Santos V., Hergueta-Redondo M., Ximénez-Embún P., Kataru R.P., Lopez A.A., Merino C., Sánchez-Redondo S., Graña-Castro O., Matei I., Nicolás-Avila J.Á., Torres-Ruiz R., Rodríguez-Perales S., Martínez L., Pérez-Martínez M., Mata G., Szumera-Ciećkiewicz A., Kalinowska I., Saltari A., Martínez-Gómez J.M., Hogan S.A., Saragovi H.U., Ortega S., Garcia-Martin C., Boskovic J., Levesque M.P., Rutkowski P., Hidalgo A., Muñoz J., Megías D., Mehrara B.J., Lyden D, & Peinado H. (2021). Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism.. Nature cancer, 2(12), 1387-1405.
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