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Donkey anti goat igg alexa fluor 488 conjugate

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Donkey anti-goat IgG Alexa Fluor 488 conjugate is a secondary antibody reagent. It is produced by conjugating donkey anti-goat IgG antibodies with Alexa Fluor 488 dye.

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Lab products found in correlation

Alexa fluor 488 donkey anti goat, by Thermo Fisher Scientific (2 mentions) Donkey anti mouse alexa fluor 555 conjugate, by Thermo Fisher Scientific (2 mentions) Donkey anti rabbit igg alexa fluor 594 conjugate, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg1 alexa fluor 555 conjugate, by Thermo Fisher Scientific (2 mentions) Donkey anti rabbit igg hrp conjugate, by GE Healthcare (2 mentions) Sheep anti mouse igg hrp conjugate, by Jackson ImmunoResearch (2 mentions) Donkey anti chicken igy alexa fluor 647 conjugate, by Jackson ImmunoResearch (2 mentions) Donkey anti rabbit igg alexa fluor 555 conjugate, by Thermo Fisher Scientific (2 mentions) Affigel 10 beads, by Bio-Rad (1 mentions) Mouse monoclonal anti acetylated tubulin antibody, by Merck Group (1 mentions) Mouse monoclonal anti tubulin, by Merck Group (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 donkey anti-goat IgG Alexa Fluor 488 donkey anti-goat Alexa 488 donkey-anti-goat IgG Alexa Fluor 488 anti-goat IgG Alexa 488 donkey-anti-goat Alexa Fluor 488 goat anti Alexa Fluor 488 conjugate Alexa Fluor 488 anti- IgG Donkey Alexa-Fluor-488) Donkey anti-goat 488

4 protocols using donkey anti goat igg alexa fluor 488 conjugate

1

Immunohistochemical Analysis of Engineered Tissues

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Engineered tissues and host auxiliary tissues were harvested and formalin-fixed, paraffin-embedded for histology. Embedded samples were sectioned, and heat-induced antigen retrieval was performed in a pressure cooker with sodium citrate buffer. Samples were blocked with 1.5% normal goat serum, followed by incubation with primary antibodies listed in Table 1 overnight at 4°C, and incubation with secondary antibodies for 1 hour at room temperature or overnight at 4°C. Secondary antibodies used were donkey anti-goat Alexa Fluor 488 (Invitrogen), donkey anti-goat IgG Alexa Fluor 488 conjugate (Invitrogen), donkey anti-mouse Alexa Fluor® 555 conjugate (Invitrogen), donkey anti-rabbit IgG Alexa Fluor 594 conjugate (Novex), and goat anti-mouse IgG1 Alexa Fluor 555 conjugate (Novex). Nuclei were counterstained with Hoechst or DAPI. Immunohistochemical detection of human mitochondria was performed using the mouse-specific HRP/DAB (ABC) Detection IHC Kit (ab64259). Images were acquired using the microscopes specified in the following section.
Poupart J, & Waddell C.S. (2000). The rib1 Mutant Is Resistant to Indole-3-Butyric Acid, an Endogenous Auxin in Arabidopsis. Plant Physiology, 124(4), 1739-1751.
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2

Immunohistochemical Analysis of Engineered Tissues

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Engineered tissues and host auxiliary tissues were harvested and formalin-fixed, paraffin-embedded for histology. Embedded samples were sectioned, and heat-induced antigen retrieval was performed in a pressure cooker with sodium citrate buffer. Samples were blocked with 1.5% normal goat serum, followed by incubation with primary antibodies listed in Table 1 overnight at 4°C, and incubation with secondary antibodies for 1 hour at room temperature or overnight at 4°C. Secondary antibodies used were donkey anti-goat Alexa Fluor 488 (Invitrogen), donkey anti-goat IgG Alexa Fluor 488 conjugate (Invitrogen), donkey anti-mouse Alexa Fluor® 555 conjugate (Invitrogen), donkey anti-rabbit IgG Alexa Fluor 594 conjugate (Novex), and goat anti-mouse IgG1 Alexa Fluor 555 conjugate (Novex). Nuclei were counterstained with Hoechst or DAPI. Immunohistochemical detection of human mitochondria was performed using the mouse-specific HRP/DAB (ABC) Detection IHC Kit (ab64259). Images were acquired using the microscopes specified in the following section.
Fortin C.L., McCray T.N., Saxton S.H., Johansson F., Andino C.B., Mene J., Wang Y, & Stevens K.R. (2022). Temporal dynamics of metabolic acquisition in grafted engineered human liver tissue. Advanced biology, 7(5), e2200208.
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3

Immunoblotting and Immunofluorescence Antibody Protocols

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Mouse monoclonal anti-FLAG-M1 and mouse monoclonal anti–human protein C (HPC) antibodies were a generous gift from Andrew Kruse (Harvard Medical School). Mouse monoclonal anti-SHH antibody (clone 5E1) (Ericson et al., 1996 (link)) was obtained from the Developmental Studies Hybridoma Bank (University of Iowa). Primary antibodies for immunoblotting were used at 1–2 μg/mL, in TBST [10 mM Tris-HCl, pH 7.6; 150 mM NaCl; 0.2% (v/v) Triton X-100] with 5% (w/v) non-fat dry milk. In experiments using calcium-dependent anti-FLAG-M1 and anti-HPC antibodies, all buffers were supplemented with 2 mM CaCl2. Secondary antibodies used for immunoblotting (0.2 μg/mL final concentration) were: sheep anti-mouse IgG–HRP conjugate (Jackson ImmunoResearch) and donkey anti-rabbit IgG–HRP conjugate (GE Healthcare). Primary and secondary antibodies for immunofluorescence were used at 1 μg/mL, in TBST with 5% (w/v) bovine serum albumin (BSA). Immunofluorescence secondary antibodies were: donkey anti-chicken IgY–Alexa Fluor 647 conjugate (Jackson ImmunoResearch), donkey anti-rabbit IgG–Alexa Fluor 555 conjugate (Thermo), and donkey anti-goat IgG–Alexa Fluor 488 conjugate (Thermo).
Huang P., Wierbowski B.M., Lian T., Chan C., García-Linares S., Jiang J, & Salic A. (2022). Structural basis for catalyzed assembly of the Sonic hedgehog–Patched1 signaling complex. Developmental cell, 57(5), 670-685.e8.
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4

Antibody Characterization for Ciliary Research

Cited 2 times
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The following antibodies were purchased: rabbit anti-SHH monoclonal antibody (Cell Signaling Technology), rabbit anti-WNT3A monoclonal antibody (Cell Signaling Technology), rabbit anti-GFP polyclonal antibody (Rockland), mouse monoclonal anti-tubulin (Sigma), mouse anti-acetylated tubulin monoclonal antibody (Sigma), rat anti-HA–HRP conjugate (Roche), sheep anti-mouse IgG–HRP conjugate (Jackson ImmunoResearch), donkey anti-rabbit IgG–HRP conjugate (GE Healthcare), donkey anti-goat IgG–Alexa Fluor 488 conjugate (Thermo), donkey anti-rabbit IgG-Alexa Fluor 555 conjugate (Thermo), and donkey anti-chicken IgY–Alexa Fluor 647 conjugate (Jackson ImmunoResearch). Antibodies against mCherry (Nedelcu et al., 2013 (link)) and against mouse SMO (Tukachinsky et al., 2010 (link)) were described previously. Polyclonal antibodies against mouse ARL13B (mARL13B), a marker of cilia, were raised in chickens. Briefly, 6xHis-tagged mARL13B was expressed in E. coli (BL21 DE3 pLysS, Novagen), was purified as a soluble protein, and was used to immunize hens (Abcore). Whole IgY was isolated from eggs laid by the immunized hens, as described (Pauly et al., 2011 ). Anti-mARL13B antibodies were isolated from whole IgY, by affinity purification against recombinant MBP-tagged mARL13B immobilized on Affi-Gel 10 beads (Bio-Rad).
Petrov K., Wierbowski B.M., Liu J, & Salic A. (2020). Distinct cation gradients power cholesterol transport at different key points in the Hedgehog signaling pathway. Developmental cell, 55(3), 314-327.e7.
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