Cytoseal xyl

Manufactured by Epredia

Cytoseal XYL is a xylene-based mounting medium designed for use in histological and cytological applications. It is a clear, colorless solution that is miscible with alcohol and other organic solvents. Cytoseal XYL is used to permanently mount and protect microscope slides containing tissue or cell samples.

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Lab products found in correlation

Wright giemsa stain, by Merck Group (1 mentions) Nanozoomer s360, by Hamamatsu Photonics (1 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Histoclear, by National Diagnostics (1 mentions)

3 protocols using cytoseal xyl

Isolation and Analysis of Granulocyte-Macrophage Progenitors

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WT or VDR^–/– cells from the colony-forming assays were isolated via dissolution of the semisolid media. Colonies were removed by washing with IMDM + 2% FBS. Cells were centrifuged, and the media was decanted via aspiration; this wash step was repeated twice. Isolated cells were stained for FACS to isolate GMPs. GMPs were subjected to FACS using phosphate-buffered saline (PBS) + 3% bovine serum albumin and spun onto microscope slides. The slides were placed in 98% methanol for 7 minutes to fix the cells. Cells were stained with 0.02% Wright-Giemsa stain (Sigma-Aldrich) for 30 minutes and washed with distilled water to remove excess stain. Slides were mounted in Cytoseal XYL (Epredia) and coverslipped. Images were obtained with the NanoZoomer S360 digital scanner (Hamamatsu). Images were uploaded to the PathCore database, and representative images were selected using the PathCore Flow solution.

Thompson B., Lu S., Revilla J., Uddin M.J., Oakland D.N., Brovero S., Keles S., Bresnick E.H., Petri WA J.r, & Burgess S.L. (2023). Secondary bile acids function through the vitamin D receptor in myeloid progenitors to promote myelopoiesis. Blood Advances, 7(17), 4970-4982.

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Quantifying Human CD46 Expression

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Five-micrometer sections were deparaffinized by series of washes in xylene and hydrated in decreasing concentrations of ethanol washes according to standard protocols. BaseScope assay (ACDBio, Newark, CA) was performed following the standard protocol published by the manufacturer using a custom 1ZZ probe designed to target nucleotides 360–410 of human CD46 (GenBank: NM_172351.3) and not to cross detect endogenous mouse transcripts. Chromogenically developed sections (fast red) were counterstained with hematoxylin (VectorLabs, Newark, CA) and coverslipped with Cytoseal XYL (Epredia, Kalamazoo, MI). Tiled RGB photomicrographs were captured from whole-mount sections under ×10 magnification via brightfield microscopy (DM6 – Leica, Wetzlar, Germany).

Kul E., Okoroafor U., Dougherty A., Palkovic L., Li H., Valiño-Ramos P., Aberman L, & Young SM J.r. (2024). Development of adenoviral vectors that transduce Purkinje cells and other cerebellar cell-types in the cerebellum of a humanized mouse model. Molecular Therapy. Methods & Clinical Development, 32(2), 101243.

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Histological Analysis of Tumor Tissues

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Dry tissue slides from control, RFA and non-RFA treated tumor tissues from tumor-bearing mice and resected samples from PDAC patients were soaked in Histoclear (National Diagnostics, cat. HS-200) for 8 min and gradually rehydrated in decreasing concentrations of alcohol (Fisher Bioreagents, cat. BP28184) 100% and 95% for 4 min each. Slides were then rinsed in the following solutions for 3 min each: tap water, Millipore Sigma 65067-75 HARLECO^® Hematoxylin (cat. EM-65067-75), tap water, Epredia^™ Signature Series Clarifier^™ (Thermo Scientific, cat. 22-050-117), tap water, Scott’s Bluing Reagent (Polysciences, cat. 24605-1) and tap water. A counterstain was performed by 95% EtOH for 1 min followed by Eosin-Y stain (Richard-Allan Scientific, cat. #71311) and a final dehydration step was performed in increasing concentrations of alcohol 95% for 1 min, followed by 100% for 4 min and Histoclear for 4 min. Slides were mounted in Cytoseal XYL (Epredia, cat. 8312-4) mounting media.

Faraoni E.Y., O'Brien B.J., Strickland L.N., Osborn B.K., Mota V., Chaney J., Atkins C.L., Cen P., Rowe J., Cardenas J., Poulsen K.L., Wray C.J., Thosani N.C, & Bailey-Lundberg J.M. (2022). Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer. Cancer Immunology Research, 11(1), 4-12.

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