High fidelity pcr enzyme 2 phanta max master mix

Manufactured by Vazyme

Sourced in China

Vazyme's High-fidelity PCR enzyme-2× Phanta Max Master Mix is a ready-to-use solution for high-fidelity PCR amplification. It contains a novel high-fidelity DNA polymerase and optimized buffer components to provide efficient and accurate DNA synthesis.

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Lab products found in correlation

Cell counting kit cck 8, by Yeasen (3 mentions) Luciferase detection kit, by Promega (2 mentions) Plasmid extraction kit, by Tiangen Biotech (2 mentions) Gel extraction kit, by CWBIO (2 mentions) Pmd18 t 6011, by Takara Bio (2 mentions) Cell genome extraction kit, by Tiangen Biotech (2 mentions) Taq master mix, by Vazyme (2 mentions) Tunel apoptosis detection kit, by Yeasen (1 mentions) Rabbit anti sars cov 2 np antibody, by Sino Biological (1 mentions) Luciferase, by Promega (1 mentions) Pmd18 t, by Takara Bio (1 mentions) Anti hdac1, by Abcam (1 mentions) Anti hdac2, by Abcam (1 mentions) Anti h3k18ac, by Abcam (1 mentions) Recombinant human ifn α, by R&D Systems (1 mentions) Anti yy1, by Cell Signaling Technology (1 mentions) Recombinant human ifn γ, by R&D Systems (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Goat anti mouse igg hrp, by Proteintech (1 mentions) Anti myc, by ABclonal (1 mentions)

3 protocols using high fidelity pcr enzyme 2 phanta max master mix

Antibody Sources for SARS-CoV-2 Research

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The following antibodies were used throughout this study: from Abclonal (Wuhan, China), anti-FLAG (AE063), anti-HA (AE036), anti-ASGR1 (A13279), anti-β-Actin (AC026). From Abcam (Cambridge, UK), anti-ASGR1 (ab254262). From proteintech (Wuhan, China), anti-ACE2 (21115-1-AP). From Sino Biological (Beijing, China), rabbit-anti-SARS-CoV-2 NP antibody. From R&D systems (UMN, USA), anti-His-Alexa Fluor488-conjugated Antibody (IC050G). Anti-Flag Magnetic Beads (HY-K0207) and ACE2/ANG (1-7) inhibitor-A779 (HY-P0216) were purchased from MCE. The Sipke of SARS-CoV-2 (DRA59) was purchased from novoprotein (Shanghai, China). 2× Taq Master Mix (P112) and High-fidelity PCR enzyme-2× Phanta Max Master Mix (P515) were purchased from Vazyme (Nanjing, China). PMD18-T (6011) was purchased from Takara (Beijing, China). Cell Genome Extraction Kit (DP304) and Plasmid Extraction Kit (DP103, DP108, DP117) were purchased from Tiangen (Beijing, China). Gel Extraction Kit (CW2302) was purchased from CWBIO (Nanjing, China). Luciferase detection kit (E6110) was purchased from Promega (Madison, USA). Cell Counting Kit (CCK-8) and TUNEL Apoptosis Detection Kit (FITC) were purchased from Yeasen (Shanghai, China).

Yang X., Zheng X., Zhu Y., Zhao X., Liu J., Xun J., Yuan S., Chen J., Pan H., Yang J., Wang J., Liang Z., Shen X., Liang Y., Lin Q., Liang H., Li M., Peng F., Lu D., Xu J., Lu H., Jiang S., Zhao P, & Zhu H. (2024). Asialoglycoprotein receptor 1 promotes SARS-CoV-2 infection of human normal hepatocytes. Signal Transduction and Targeted Therapy, 9, 42.

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Antibody Characterization and Validation

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The following antibodies were used throughout this study: anti-FKBP3 from Thermo Fisher (catalog no. PA5-19483); anti-HDAC1 (catalog no. ab280198), anti-HDAC2 (catalog no. ab219053), anti-H3K4ac (catalog no. ab176799), and anti-H3K18ac (catalog no. ab40888) from Abcam (Cambridge, UK); and anti-YY1 (catalog no. 46395) and anti-β-actin (catalog no. 4970) from Cell Signaling Technology (MA, USA). We purchased 2× Taq master mix (catalog no. P112) and high-fidelity PCR enzyme 2× Phanta Max master mix (catalog no. P515) from Vazyme (Nanjing, China). PMD18-T (catalog no. 6011) was purchased from TaKaRa (Beijing, China). Cell genome extraction kit (catalog no. DP304) and plasmid extraction kit (catalog nos. DP103, DP108, and DP117) were purchased from Tiangen (Beijing, China). Gel extraction kit (catalog no. CW2302) was purchased from CWBio (Nanjing, China). Luciferase and NanoLuc detection kits (catalog nos. E6110 and N1110, respectively) were purchased from Promega (Madison, USA). Recombinant human IFN-α (catalog no. 11200-1), recombinant human IFN-β (catalog no. 8499-IF), and recombinant human IFN-γ (catalog no. 285-IF) were purchased from R&D Systems (USA). Cell counting kit (CCK-8) and TUNEL apoptosis detection kit (fluorescein isothiocyanate [FITC]) were purchased from Yeasen (Shanghai, China).

Yang X., Zhao X., Zhu Y., Shen Y., Wang Y., Lu P., Jiang Z., Pan H., Yang J., Xun J., Zhao L., Wang J., Liang Z., Shen X., Liang Y., Lin Q., Liang H., Jin L., Zhang D., Liu J., Wang B., Jiang S., Xu J., Wu H., Lu H, & Zhu H. (2021). FKBP3 Induces Human Immunodeficiency Virus Type 1 Latency by Recruiting Histone Deacetylase 1/2 to the Viral Long Terminal Repeat. mBio, 12(4), e00795-21.

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Antibody and Reagents Utilized for Research

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The following antibodies were used throughout this study: from ABclonal(Wuhan, China), anti-FLAG (AE063; 1:2500 for Western blot), anti-Myc (AE070; 1:2500 for Western blot), anti-β-Actin (AC026; 1:5000 for Western blot). From Abcam (Cambridge, UK), anti-hnRNP U (ab264142; 1:1000 for Western blot). From Proteintech (Wuhan, China), HRP goat anti-mouse IgG (15014; 1:5000 for Western blot), HRP goat anti-rabbite IgG (15015; 1:5000 for Western blot). anti-FLAG Magnetic Beads (HY-K0207) was purchased from MCE. 2 × Taq Master Mix (P112), High fidelity PCR enzyme- 2 × Phanta Max Master Mix (P515) were purchased from Vazyme (Nanjing, China). PMD18-T (6011) was purchased from Takara (Beijing, China). Cell Genome Extraction Kit (DP304), Plasmid Extraction Kit (DP103, DP108, DP117) were purchased from Tiangen (Beijing, China). Gel Extraction Kit (CW2302) was purchased from CWBIO (Nanjing, China). Luciferase detection kit (E6110) was purchased from Promega (Madison, USA). Cell Counting Kit (CCK-8) and TUNEL-Alexa Flour 640 apoptosis kit were purchased from Yeasen (Shanghai, China). Luciferase and nanoluc detection kit (E6110, N1110) was purchased from Promega (Madison, USA). Recombinant human IFN-alpha (11200-1), recombinant human IFN-beta (8499-IF) and recombinant human IFN-gamma (285-IF) were purchased from R&D Systems (UN).

Yang X., Zhao X., Zhu Y., Xun J., Wen Q., Pan H., Yang J., Wang J., Liang Z., Shen X., Liang Y., Lin Q., Liang H., Li M., Chen J., Jiang S., Xu J., Lu H, & Zhu H. (2022). FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation. Emerging Microbes & Infections, 11(1), 2785-2799.

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