Anti γδ tcr

Manufactured by Beckman Coulter

The Anti-γδ TCR is a laboratory reagent used to detect and identify gamma-delta T cells in biological samples. It binds specifically to the gamma-delta T cell receptor, allowing for the identification and enumeration of this T cell subset.

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Lab products found in correlation

Anti cd107a, by Thermo Fisher Scientific (1 mentions) Monensin, by Thermo Fisher Scientific (1 mentions) Anti cd45ra 2h4, by Beckman Coulter (1 mentions) Anti cd45ro, by Beckman Coulter (1 mentions) Navios, by Beckman Coulter (1 mentions) 7 aminoactinomycin d, by Beckman Coulter (1 mentions)

Spelling variants of this product

Anti‐TCR‐pan‐γδ (Immu510) (2 citations) Anti-γδ TCR Ab (1 citations)

2 protocols using anti γδ tcr

Measuring γδ NKT Cell Degranulation and Granzyme B Secretion

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To study the degranulation of γδ NKT cells, γδ NKT cells were coculture with various target cells at an E:T ratio of 1 in the presence of anti-CD107a (eBioscience), anti-CD107b (eBioscience) and 2 μM monensin (eBioscience). After 2-hour incubation, samples were stained with anti-γδ TCR (Beckman Coulter) and analyzed with flow cytometer.
To measure GrB secretion, a Human Granzyme B ELISpot Kit (R&D Systems, Minneapolis, MN, https://www.rndsystems.com) was used. In brief, 0 to 10⁴ γδ NKT cells were incubated with 5×10⁴ various cancer cells on a human GrB microplate for 4 hours. GrB spots were then stained as described in the manufacturer’s manual and counted with an ImmunoSpot Analyzer (CTL, Shaker Heights, OH, http://www.immunospot.com).

Zeng J., Tang S.Y, & Wang S. (2019). Derivation of mimetic γδ T cells endowed with cancer recognition receptors from reprogrammed γδ T cell. PLoS ONE, 14(5), e0216815.

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Multiparametric Flow Cytometry Phenotyping

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Cells were harvested and phenotypically characterized by 10-color flow cytometry (Navios, Beckman Coulter) using the following antibodies: anti-CD45 (J.33), anti-CD14 (RMO52), anti-CD3 (UCHT-1), anti-CD56 (N901), anti-CD19 (J3-119), anti-CD16 (3G8), anti-CD4 (13B8.2), anti-CD8 (B9.11), anti-CD25 (B1.49.9), anti-CD314 (ON72), anti-αβ-TCR (BW242/412), anti-γδ-TCR (IMMU510), anti-CD45RO (UCHL1), anti-CD45RA (2H4) and anti-CD62L (DREG56) (Beckman Coulter; except antiαβ-TCR, Miltenyi Biotec). For the assessment of viability, 7-aminoactinomycin D (Beckman Coulter) was used, and measurements were performed using a single-platform approach. EBV-specific lymphocytes were identified by staining with various MHC-I and MHC-II antibodies using multimer technology: LMP2A (A*02:01), EBNA1 (B*35:01), EBNA3A (B*07:02 and B*08:01), BMLF1 (A*02:01; Immudex) and EBNA1 (DRB1*04:01; ProImmune).

Pfeffermann L.M., Pfirrmann V., Huenecke S., Bremm M., Bonig H., Kvasnicka H.M., Klingebiel T., Bader P, & Rettinger E. (2018). Epstein-Barr virus-specific cytokine-induced killer cells for treatment of Epstein-Barr virus-related malignant lymphoma. Cytotherapy, 20(6).

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