Frozen sections (3 μm) were prepared for MitoTracker deep red staining (M22426; Thermo Fisher, USA) according to the manufacturer's instructions to assess mitochondrial mass. Images were captured by confocal microscopy (Leica TCS SP2 AOBS; Leica Microsystems, Buffalo Grove, IL, USA). Frozen sections (3 μm) or cells cultured on coverslips were prepared for senescence-associated β-galactosidase (SA-β-gal) staining (#9860; Cell Signaling Technology) according to the protocols to assess renal senescence. Images were taken by the DP27-CU camera coupled to BX-43F microscope (Olympus, Japan).
Mitotracker deep red staining
Manufactured by Thermo Fisher Scientific
Sourced in United States
MitoTracker Deep Red is a fluorescent dye that stains mitochondria in live cells. It is cell-permeant and accumulates in active mitochondria. The dye can be used to visualize mitochondria and monitor mitochondrial function.
Automatically generated - may contain errors
2 protocols using mitotracker deep red staining
1
Mitochondrial Mass and Senescence Evaluation
2
Histological Assessment of Calf Muscle
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Isolated calf muscles were fixed in 10% formalin overnight at 4 °C followed by replacement with 70% EtOH and maintaining at 4 °C minimum overnight. Tissues were processed with paraffin embedding, sectioning, and placed on slides. Dewaxed sections were processed with regular hematoxylin and eosin staining. For Oil red O staining, isolated calf muscle was snap frozen in liquid nitrogen and then stored in −80 °C. Tissues were embedded in OTC compound and sectioned with Cryostat in 12 μm thickness and placed on slides. Sections on slides were rounded with liquid blocker pen. Sections were covered with Oil red O solution and incubated at room temperature for 10 min. Then slides were rinsed with running tap water for 30 min. Sections were captured as bright-field images with a light microscope. MitoTracker deep red staining (Thermo Fisher) was performed based on the manufacturer’s protocol.
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