Anti cox 2 antibody

Manufactured by Thermo Fisher Scientific

The Anti-COX-2 antibody is a laboratory reagent used for the detection and quantification of cyclooxygenase-2 (COX-2) in various sample types. COX-2 is an enzyme involved in the production of prostaglandins, which play a role in inflammation and other physiological processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) to measure COX-2 levels in cells, tissues, or biological fluids.

Automatically generated - may contain errors

Lab products found in correlation

Anti oxa1 antibody, by Santa Cruz Biotechnology (2 mentions) Anti hog1 antibody, by Santa Cruz Biotechnology (2 mentions) Bx61 microscope, by Olympus (1 mentions) 7900ht fast system, by Thermo Fisher Scientific (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) Sybr green qpcr mastermix, by Qiagen (1 mentions) Il 1β, by Qiagen (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Trans blot membrane, by Bio-Rad (1 mentions) Anti ha antibody, by Merck Group (1 mentions) Trans blot nitrocellulose membrane, by Bio-Rad (1 mentions)

Spelling variants of this product

COX-2 (40 citations) Anti-COX-2 (8 citations) COX-2 antibody (2 citations)

4 protocols using anti cox 2 antibody

Immunohistochemical Analysis of Gastric Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gastric tissues were post fixed in 4% paraformaldehyde overnight at 4 °C before routine paraffin sections (4 μm) were prepared for immunohistochemical staining. Paraffin-embedded slices of tissues were incubated overnight at 4 °C with primary rabbit anti-VEGF and anti-COX-2 antibody (1:200, Thermo). After rinsing, sections were incubated in biotinylated goat anti-rabbit IgG (1:200, Maixin) at 37 °C for 30 min and were subsequently incubated in streptavidin-peroxidase conjugate (1:200, Maixin) at 37 °C for 30 min. The sections were developed with 3,3′-diaminobenzidine (DAB) in a chromogen solution and counter-stained with hematoxylin. Images were obtained using an Olympus BX-61microscope (Tokyo, Japan).

Wei B., Wang Y., Wu H., Liu M., Yao W, & Wei M. (2019). Pharmacodynamics and Pharmacokinetics of a New Type of Compound Lansoprazole Capsule in Gastric Ulcer Rats and Beagle Dogs: Importance of Adjusting Oxidative Stress and Inflammation. Pharmaceutics, 11(2), 49.

+ Open protocol

+ Expand

Macrophage Activation and Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Resident peritoneal macrophages were isolated from WT or Atf3-deficient mice in DPBS^−/− and incubated for 1h in 24-well plates at 37°C in DMEM containing 10% FBS. Non-adherent cells were removed and the macrophages were either left untreated or stimulated with yeast zymosan (50µg/ml) for 3 or 6h. Supernatants were collected for LC-MS/MS analysis (see below) and cell pellets were used for gene expression analysis or Western blot. For this, RNA was extracted using the RNeasy mini kit (Qiagen), followed by cDNA synthesis. RT-PCR amplification was performed with SYBR-green qPCR master mix (SA Biosciences) using a 7900HT fast system (Applied Biosystems) and commercially available primers for murine Atf3 (IDT), Ptgs2, Ptgs1, microsomal prostaglandin E synthase 1 (mPges1), hematopoietic prostaglandin D synthase (hPGDS), arachidonate 5 lipoxygenase (Alox5), Alox5 activating protein (ap) and interleukin 1 beta (Il-1β) (SA biosciences). Relative expression was determined by the 2^−ΔΔC_T method after internal normalization to Hprt. To assess COX-2 protein expression, macrophages treated without or with zymosan for 6h and lysates were prepared. Expression of COX-2 was determined using an anti-COX-2 antibody (Thermo Scientific) and was normalized for loading using an anti-β-actin antibody (Sigma).

Hellmann J., Tang Y., Zhang M.J., Hai T., Bhatnagar A., Srivastava S, & Spite M. (2015). Atf3 negatively regulates Ptgs2/Cox2 expression during acute inflammation. Prostaglandins & other lipid mediators, 0, 49-56.

+ Open protocol

+ Expand

Western Blot Immunodetection of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

From SDS-tricine-PAGE, proteins were electrotransferred onto a nitrocellulose Trans-Blot membrane (Bio-Rad) for immunodetection. Membranes were washed, blocked, and independently incubated for 4 h with the following antibodies: anti-Cox2 antibody at a 1:9000 dilution (Invitrogen; Molecular Probes), anti-Oxa1 antibody at a 1:1000 dilution, anti-Hog1 antibody at a 1:2000 dilution (Santa Cruz Biotechnology), and anti-Atp2 antibody at a 1:50,000 dilution. Alkaline phosphatase-conjugated IgGs (1:15,000 for 2 h) were used as secondary antibodies. Insoluble black–purple precipitates were formed upon addition of nitro-blue tetrazolium chloride and 5-bromo-4-chloro-3′-indolyl phosphate p-toluidine salt. Images of the immunodecorated polypeptide bands were captured in an HP Scanjet G4050. For immunodetection on previously probed membranes using a different primary antibody, membranes were stripped by incubation for 45 min at 50°C in the presence of 2% SDS, 62.5 mM Tris-HCl, pH 6.8, and 100 mM β-mercaptoethanol.

Rubalcava-Gracia D., García-Rincón J., Pérez-Montfort R., Hamel P.P, & González-Halphen D. (2019). Key within-membrane residues and precursor dosage impact the allotopic expression of yeast subunit II of cytochrome c oxidase. Molecular Biology of the Cell, 30(18), 2358-2366.

+ Open protocol

+ Expand

Western Blot Analysis of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

From SDS-tricine-PAGE, proteins were electrotransferred onto a nitrocellulose Trans-Blot membrane (Bio-Rad) for immune detection. Membranes were washed, blocked, and independently incubated for 8 h with the following antibodies: anti-Cox1 antibody at a 1:3300 dilution (MitoSciences), anti-Cox2 antibody at a 1:7500 dilution (Invitrogen; Molecular Probes), anti-Cox3 antibody at a 1:15,000 dilution (Molecular Probes), anti-Oxa1 antibody at a 1:1000 dilution, anti-Hog1 antibody at a 1:2000 dilution (Santa Cruz Biotechnology), anti-HA antibody at a 1:15,000 dilution (Sigma Aldrich), and anti-Atp2 antibody at a 1:50,000 dilution. Alkaline phosphatase (ALP)–conjugated immunoglobulin Gs (1:15,000 for 2 h) were used as secondary antibodies. Insoluble black–purple precipitates on bands were formed upon addition of nitro blue tetrazolium chloride and 5-bromo-4-chloro-3′-indolyl phosphate p-toluidine salt. Images of the immune-decorated polypeptide bands were captured in a HP Scanjet G4050. To carry out a second Western blot reaction with a different primary antibody, membranes were stripped by incubation for 45 min at 50°C in the presence of 2% SDS, 62.5 mM Tris-HCl, pH 6.8, and 100 mM β-mercaptoethanol. Band intensity was measured by densitometric analysis using the GelAnalyzer 2010a freeware.

Rubalcava-Gracia D., Vázquez-Acevedo M., Funes S., Pérez-Martínez X, & González-Halphen D. (2018). Mitochondrial versus nuclear gene expression and membrane protein assembly: the case of subunit 2 of yeast cytochrome c oxidase. Molecular Biology of the Cell, 29(7), 820-833.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!