HSCs were seeded in 6-well plates at a density of 2 × 104 cells/well and cultured overnight. After the cells were treated with Ang II and various concentrations of carvedilol for 24 hours, nuclear morphological changes and DNA fragmentation, indicating apoptotic cells, were detected with a Hoechst 33258 fluorescence staining kit (Beyotime, Shanghai, China) according to the manufacturer's protocol. Apoptotic HSCs were observed under a fluorescence microscope (Olympus, Tokyo, Japan).
Hoechst 33258 fluorescence staining kit
Manufactured by Beyotime
Sourced in China
Hoechst 33258 is a fluorescent dye used for the staining and detection of DNA in biological samples. It binds to the minor groove of double-stranded DNA, emitting a blue fluorescence when exposed to ultraviolet light. The Hoechst 33258 fluorescence staining kit provides a simple and reliable method for visualizing and quantifying DNA content in cells and tissues.
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Lab products found in correlation
2 protocols using hoechst 33258 fluorescence staining kit
1
Apoptosis Induction in Vascular Cells
2
Matrine-induced Apoptosis in Lung Cancer Cells
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A549 or H1299 cells (5×104) in DMEM containing 10% FBS were treated with matrine (0.5 mg/ml) or PBS at 37°C for 24 h. Apoptosis was detected using a Hoechst 33258 fluorescence staining kit (Beyotime Institute of Biotechnology, Haimen, China) according to the manufacturer's protocol. The effect of matrine on cellular apoptosis was also evaluated using an Annexin-V Apoptosis Detection kit FITC (ebioscience; Thermo Fisher Scientific, Inc.) based on the manufacturer's protocol and apoptosis was determined using a flow cytometer. The results of cell apoptosis were analyzed with FlowJo software (10.0.7; FlowJo LLC, Ashland, OR, USA).
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