Log In Sign up
The largest database of trusted experimental protocols

Purified formaldehyde

Manufactured by Electron Microscopy Sciences
Visit Supplier

4% purified formaldehyde is a laboratory reagent used for fixation and preservation of biological specimens. It is a clear, colorless aqueous solution of formaldehyde.

Automatically generated - may contain errors

Lab products found in correlation

Stellaris probe, by Biosearch Technologies (2 mentions) Vectashield, by Vector Laboratories (2 mentions) Quasar 670, by Biosearch Technologies (1 mentions) Quasar 570, by Biosearch Technologies (1 mentions) Stellaris rna fish hybridization buffer, by Biosearch Technologies (1 mentions)

Spelling variants of this product

4% formaldehyde (102 citations)

3 protocols using purified formaldehyde

1

RNA FISH Protocol for Cellular Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 4% purified formaldehyde (Electron Microscopy Sciences) in ribonuclease (RNase)-free PBS for 15 min at room temperature and then permeabilized with 70% ethanol in RNase-free water at 4°C for a minimum of 1 hour [21 (link)]. Cells were washed in 1 ml of wash buffer (2x saline sodium citrate [SSC] plus 10% formamide) followed by overnight hybridization with RNA FISH probes at 37°C (Stellaris® probes; LGC Biosearch Technologies) in Stellaris® RNA FISH hybridization buffer (SMF-HB1-10), followed by one change in wash buffer (30 min at 37°C), and a second wash in buffer containing 1μg/ml DAPI (10 min at 37°C) [21 (link)]. Vectashield (Vector Laboratories) was used as the mounting medium. All probes were custom designed, synthesized, and labeled with either Quasar 570® or Quasar 670® dyes by LGC Biosearch Technologies.
Stossi F., Dandekar R.D., Johnson H., Lavere P., Foulds C.E., Mancini M.G, & Mancini M.A. (2019). Tributyltin chloride (TBT) induces RXRA down-regulation and lipid accumulation in human liver cells. PLoS ONE, 14(11), e0224405.
+ Open protocol
+ Expand
2

Visualizing mRNA Expression via RNA FISH

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA FISH experiments were completed as described in (Bolt et al., 2013 (link)). Cells were fixed in 4% purified formaldehyde
(Electron Microscopy Sciences) in RNase-free phosphate-buffered saline for 15 min and then
permeabilized with 70% ethanol in RNase-free water at 4°C for 1 h. Cells were
washed in 1 ml of wash buffer (2× SSC (Ambion) plus 10% formamide) followed by four
hours of 37°C hybridization with mRNA FISH probes (dsRED2 or GREB1
Stellaris™ probes, Biosearch Technologies Inc.) in buffer (1 g of dextran sulfate,
1 ml of 20× SSC buffer, 1 ml of formamide and 8 ml of nuclease-free water) followed
by one wash in wash buffer for 30 min at 37°C, then followed by DNA staining with
DAPI for 10 min at 37°C. Finally, cells were washed in 2× SCC buffer, and
imaged.
Stossi F., Bolt M.J., Ashcroft F.J., Lamerdin J.E., Melnick J.S., Powell R.T., Dandekar R.D., Mancini M.G., Walker C.L., Westwick J.K, & Mancini M.A. (2014). Defining Estrogenic Mechanisms of Bisphenol A Analogs through High Throughput Microscopy-based Contextual Assays. Chemistry & biology, 21(6), 743-753.
+ Open protocol
+ Expand
3

RNA FISH Assay for mRNA Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed on ice in 4% purified formaldehyde (Electron Microscopy Sciences) in ribonuclease (RNase)-free PBS for 15 min, washed in PBS and then permeabilized with 70% ethanol in RNase-free water at 4°C for a minimum of 2 h. Cells were washed in 1 ml of wash buffer (2× saline sodium citrate [SSC] plus 10% formamide) followed by overnight hybridization with RNA FISH probes at 37C (Stellaris probes; LGC Biosearch Technologies) in hybridization buffer. Next, cells were rinsed in one change in wash buffer (30 min at 37°C), and a second wash in buffer containing 1 μg/ml DAPI (10 min at 37°C), as described (52 (link)) For experiments performed on poly-lysine coated coverslips, Vectashield (Vector Laboratories) was used as mounting medium, and sealed with nail polish. All probes were designed and synthesized by LGC Biosearch Technologies. Exon-probes (labeled with Quasar 570®) were custom designed against the common coding sequence of GREB1 mRNAs, using mRNA variant NM_014668.3, nts 143–457, 755–1459 as the defined target region (VSMF-2158-5). Intron probes (labeled with Quasar 670®) were designed against 7.5 kb of introns #4 to #10 (NG_029429.1). MYC probes were available as pre-designed by LGC Biosearch Technologies.
Stossi F., Dandekar R.D., Mancini M.G., Gu G., Fuqua S.A., Nardone A., De Angelis C., Fu X., Schiff R., Bedford M.T., Xu W., Johansson H.E., Stephan C.C, & Mancini M.A. (2020). Estrogen-induced transcription at individual alleles is independent of receptor level and active conformation but can be modulated by coactivators activity. Nucleic Acids Research, 48(4), 1800-1810.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!