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2 protocols using igepal co 720

1

Comprehensive Nanomedicine Protocol Synthesis

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Arsenic trioxide, Igepal CO-720, coumarin 6 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma‒Aldrich (St. Louis, MO, USA). Manganous acetate and chitosan were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). MPEG5K-PLGA20K and mPEG5K-PLA20K were purchased from Yare Biotech, Inc. (Shanghai, China). Mal-PEG5K-PLGA20K was synthesized in Hunan HuaTeng Co., Ltd. (Changsha, China). DSS6 (Ac-CDSSDSSDSSDSSDSSDSS) peptide was synthesized in Top-peptide Co., Ltd. (Shanghai, China). Hydroxyapatite (HA) was obtained from Emperor Nano Material Co., Ltd. (Nanjing, China). 1,1ʹ-Dioctadecyl-3,3,3ʹ,3ʹ-tetramethylindotricarbocyanine iodide (DiR) was purchased from Meilun Biotech Co., Ltd. (Dalian, China). CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) was purchased from Yeasen Biotech Co., Ltd. (Shanghai, China). Puromycin dihydrochloride was purchased from Beyotime Biotech Co., Ltd. (Shanghai, China). Anti-CD44-APC and anti-CD24-FITC were purchased from eBioscience (San Diego, CA, USA). MethoCult™ SF H4636 was purchased from STEMCELL Technologies Inc. (Vancouver, Canada).
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2

Synthesis of Mesoporous Silica Nanoparticles

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Tetraethyl orthosilicate (TEOS, 98%), ethanol (EtOH, 99%), and aqueous ammonia (28%) were purchased from Sinopharm Chemical Reagent Co. Ltd. Di(ethylene glycol) ethyl ether acrylate (DEGEEA, 90%), Brij S2 (Mn: 358.6), Brij S10 (Mn: 711), Brij S100 (Mn: 4670), IGEPAL CO720 (Mn: 749), CO890 (Mn: 1982), and 2-hydroxy-2-methylpropiophenone (photoinitiator, 96%) were obtained from Sigma-Aldrich. All the chemicals were used as received without further purifications.
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