Cytological analyses of SCI13D were performed with standard hematoxylin and eosin staining or with immunocytochemical probing for vimentin, smooth muscle actin, and cytokeratin AE1–AE3 (Ventana Medical Systems, Inc., Oro Valley, AZ, USA).
Cytokeratin ae1 ae3
Manufactured by Roche
Sourced in United States
Cytokeratin AE1–AE3 is a cocktail of monoclonal antibodies that recognizes various cytokeratin polypeptides. It is used for the identification of epithelial cells in histological and cytological samples.
Automatically generated - may contain errors
Lab products found in correlation
Vimentin, by Roche (1 mentions)
Brachyury, by Santa Cruz Biotechnology (1 mentions)
Tecnai g2 12, by Thermo Fisher Scientific (1 mentions)
Melan a, by Agilent Technologies (1 mentions)
Hmb45, by Agilent Technologies (1 mentions)
Collagen 4, by Agilent Technologies (1 mentions)
Fli 1, by BD (1 mentions)
Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions)
2 protocols using cytokeratin ae1 ae3
1
Cytological Analyses of SCI13D
2
Comprehensive Immunohistochemical and Electron Microscopic Analysis
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Immunohistochemistry was performed using commercially available antibodies to Collagen IV, CD31, GFAP, HMB45, Melan-A (Dako; Carpinteria, CA), S100, cytokeratin AE1/AE3, Ki67 (Ventana; Tucson, AZ), brachyury (Santa Cruz; Dallas, TX), FLI-1, and Cam 5.2 (BD Pharmingen; San Jose, CA). Fixed tissue was processed for electron microscopy, embedded in epoxy resin, cut for light microscopy sections, and subsequently for ultrathin sections using an ultramicrotome. Ultrathin sections were cut at 100 nm, mounted on copper grids, stained differentially using lead citrate, and examined in an FEI Tecnai G 2 12 transmission electron microscope. Digital images were captured with an AMT side mount CCD camera system.
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