Silver enhancement kit

Manufactured by Merck Group

Sourced in United States, United Kingdom

The Silver Enhancement Kit is a laboratory tool used to amplify the visibility of nano-scale structures or particles that have been labeled with a gold or silver probe. The kit contains all the necessary components to enhance the signal from the labeled targets, allowing for improved detection and imaging.

Automatically generated - may contain errors

Lab products found in correlation

Tissue tek oct, by Sakura Finetek (1 mentions) Tween 20, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Caveolin 1 antibody, by Cell Signaling Technology (1 mentions)

3 protocols using silver enhancement kit

Detecting NPAuG3-S8 in CD1 Mouse Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the presence of NPAuG3-S8 in the brains of CD1 mice, the same four randomized CD1 mice groups were used. Mice were injected intravenously in the tail vein with 3.5 mg/Kg of NPAuG3-S8 dissolved in PBS and mice were sacrificed at 10 h, 24 h and 48 h after the inoculation. Then, the brain of each mouse was extracted and was flashed frozen in Tissue-Tek O.C.T. (Sakura Finetek, Torrance, California, USA).
For cryogenic sections, slides with histological sections were incubated in 0.1% Triton X-100 for 5 min and washed. NPAuG3-S8 was stained by the silver enhancement kit (Sigma, St. Louis, Missouri, USA) for 5 min, washed and fixed with 2.5% sodium thiosulfate solution (STS). Counterstain with eosin and mounts of histological sections were performed in order to determine the presence of NP by light microscopy.

I R.I., MJ S., R G., FJ D.L., MJ B, & MA M.F. (2020). Gold Nanoparticles Crossing Blood-Brain Barrier Prevent HSV-1 Infection and Reduce Herpes Associated Amyloid-βsecretion. Journal of Clinical Medicine, 9(1), 155.

+ Open protocol

+ Expand

Biotin-Based Sandwich Immunoassay for PSA

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified anti-human IL-1β biotin and anti-human IL-1β were supplied by e-Biosciences (Hatfield, UK). Anti-PSA CapAb and anti-PSA DetAb conjugated with gold nanoparticles were supplied by Wama Diagnóstica (São Carlos, Brazil). The silver enhancement kit was supplied by Sigma-Aldrich (Dorset, UK). The PSA native protein was purchased from Abcam (Cambridge, UK). The Carbon nanoparticles were supplied by Wageningen Food and Biobased Research, Wageningen University & Research (Wageningen, The Netherlands). The particles cluster to irregular shapes with an average size of 150 to 200 nm.
Phosphate-buffered saline (PBS) and bovine serum albumin (BSA) were sourced from Sigma-Aldrich (Dorset, UK). PBS pH 7.4, 10 mM was used as the main experimental buffer. The blocking solution consisted of 3% w/v protease-free BSA diluted in PBS buffer. For washings, PBS with 0.05% v/v of Tween-20 (Sigma-Aldrich, Dorset, UK) was used. The FEP-Teflon microcapillary film (MCF) is fabricated from fluorinated ethylene propylene co-polymer by melt-extrusion process by Lamina Dielectrics Ltd. (Billingshurst, West Sussex, UK). FEP-Teflon MCF presents 10 bore parallel capillaries with 223 ± 23-μm diameter.

Barbosa A.I., Wichers J.H., van Amerongen A, & Reis N.M. (2017). Towards One-Step Quantitation of Prostate-Specific Antigen (PSA) in Microfluidic Devices: Feasibility of Optical Detection with Nanoparticle Labels. Bionanoscience, 7(4), 718-726.

+ Open protocol

+ Expand

Caveolin-1 Immunogold Labeling in Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Caveolin-1 immuno-EM labeling was performed as described previously (Andreone et al., 2017) . Mice were anesthetized and perfused through the heart with 30 mL PBS, followed by first 150 mL of a 5% glutaraldehyde/4% PFA/0.1 mM phosphate buffer fixative solution, then a 4% PFA/0.1 mM phosphate buffer fixative solution. Brains were dissected and post-fixed in 4% PFA/PBS for 30 min at 4 C. Coronal vibratome free-floating sections of 50 mm were collected and immersed in 0.1% sodium borohydride/PBS for 20 min at room temperature, blocked with 10% goat serum/PBST (0.01% Triton X-100), and incubated with Caveolin-1 antibody (1:100, Cell Signaling Technologies. 3267) overnight at room temperature. Sections were then incubated with gold-labeled goat antirabbit IgG (1:50, Nanoprobes. 2003) overnight at room temperature, washed with PBS and sodium acetate, and silver-enhanced using Silver Enhancement kit (Sigma, SE100) prior to processing for TEM. Data were collected from 4 mice per group (8 capillary cross-sections per mouse) and analyzed in a double-blind fashion. Randomization procedures are not applicable to these experiments. G-power software was used for sample size estimation. No data were excluded.

Park M.H., Lee J.Y., Park K.H., Jung I.K., Kim K.T., Lee Y.S., Ryu H.H., Jeong Y., Kang M., Schwaninger M., Gulbins E., Reichel M., Kornhuber J., Yamaguchi T., Kim H.J., Kim S.H., Schuchman E.H., Jin H.K, & Bae J.S. (2018). Vascular and Neurogenic Rejuvenation in Aging Mice by Modulation of ASM. Neuron, 100(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!