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Recombinant standard proteins

Manufactured by Thermo Fisher Scientific
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Recombinant standard proteins are laboratory reagents used as reference materials for various analytical and testing applications. They are produced through recombinant DNA technology and serve as benchmarks to calibrate, validate, and optimize experimental protocols.

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Lab products found in correlation

Griess reaction, by Merck Group (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions)

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Protein standards (14 citations) Recombinant proteins (5 citations) Standards for recombinant cytokine proteins (2 citations)

2 protocols using recombinant standard proteins

1

Quantifying Rat IFN-γ and Nitric Oxide

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Assessment and quantification of rat IFN-γ in supernatants recovered from in vitro co-cultures was performed using standard sandwich ELISAs, according to the manufacturer’s instructions (eBioscience, San Diego, CA); IFN-γ was quantified by comparison to recombinant standard proteins (Peprotech, Rocky Hill, NJ). Estimation of nitric oxide was performed in culture supernatants using the Griess reaction (Sigma-Aldrich, St Louis, MO) and quantified by comparison to serially diluted NaNO2 [25 ].
De Pascalis R., Hahn A., Brook H.M., Ryden P., Donart N., Mittereder L., Frey B., Wu T.H, & Elkins K.L. (2018). A panel of correlates predicts vaccine-induced protection of rats against respiratory challenge with virulent Francisella tularensis. PLoS ONE, 13(5), e0198140.
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2

Multiplex Assay Reagent Preparation

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Cell culture reagents were purchased from Fisher Scientific. MagPlex® magnetic microspheres (beads) were from Luminex Corp. Recombinant standard proteins were purchased from Peprotech and Bio-techne. Buffers for cell lysis and multiplex assays were prepared in-house using reagents from Sigma-Aldrich and Santa Cruz Biotechnology.
Tsolakos N., Haswell L.E., Miazzi F., Bishop E., Antoranz A., Pliaka V., Minia A., Alexopoulos L.G., Gaca M, & Breheny D. (2024). Comparative toxicological assessment of cigarettes and new category products via an in vitro multiplex proteomics platform. Toxicology Reports, 12, 492-501.
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