All experiments were performed in the absence of serum (i.e. serum-free RPMI or DMEM-F12). Cells were plated overnight in complete medium, to be replaced with serum- free medium for the next 72 hours. The P2×7R inhibitor AZ10606120 (Tocris Bioscience, Ellisville, MS, USA) was dissolved as a stock solution of 100 mM in DMSO, and used for all in vitro and in vivo experiments at a final concentration of 300 nM in sterile PBS (EuroClone).
Sterile pbs
Manufactured by Euroclone
Sourced in United States
Sterile PBS is a pre-prepared phosphate-buffered saline solution. It is a sterile, isotonic buffer that maintains a physiological pH. Sterile PBS is commonly used as a diluent or washing solution in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Az10606120, by Bio-Techne (1 mentions)
Human fibronectin, by Merck Group (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Euroclone (1 mentions)
Antibiotic antimycotic, by Euroclone (1 mentions)
Dmem high glucose, by Euroclone (1 mentions)
L glutamine, by Euroclone (1 mentions)
2 protocols using sterile pbs
1
P2X7R Inhibitor Protocol
2
Cell Culture and Preconditioning Methods
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse muscle myoblast cells (C2C12) were expanded in medium consisting in DMEM high glucose (Euroclone), 10% Fetal Bovine serum (Euroclone), 1% antibiotic/antimycotic (Euroclone), 1% L-glutamine (Euroclone), and 1% sodium Pyruvate (GIBCO). Cells were cultured in 75 cm 2 flask at 37°C and 5% CO 2 in humidified atmosphere and expanded until 70% confluence was reached. For this experiment the cell passage was 16. Samples were pretreated by two different methods -one group was preconditioned with complete expansion medium at 37°C for 30 minutes, the other was incubated with human fibronectin (Sigma Aldrich) for 1 hour at 37°C. As a control silk fibroin sheets without cuts were used and preconditioned with complete expansion medium and with human fibronectin, as kirigami films. All samples were sterilized with 70% ethanol for 40 minutes and then washed twice with sterile PBS (Euroclone).
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