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2 protocols using rabbit polyclonal anti src antibody

1

Protein Expression and Phosphorylation Analysis

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Primary: rabbit polyclonal anti-CDK/2 (M2), cat no. sc-1632 Santa Cruz Biotechnologies; mouse monoclonal (9E10) anti-c-Myc, cat no. SC-40 Santa Cruz Biotechnologies; rabbit polyclonal anti-p-c-Myc (Thr58/Ser 62), cat no. Sc-8000R, Santa Cruz Biotechnologies; mouse monoclonal anti-SCML2 (SCMAD14a), cat no. ab51506 Abcam; rabbit polyclonal antip-Src (Tyr416), cat no. 2101S, Cell Signaling; rabbit polyclonal anti-Src antibody, cat no. 2108S, Cell Signaling, rabbit monoclonal anti-p27 Kip1 (D69C12) XP® cat no. 3686; Santa Cruz Biotechnology; mouse monoclonal p21 (F-5), cat no. sc-6246, Santa Cruz Biotechnologies; mouse monoclonal anti Nanog, clone 7F7-1, cat no. MABD24, EMD Millipore; rabbit polyclonal anti-Bmi-1 antibody, cat no. ab38295, Abcam. Mouse monoclonal anti-tubulin, cat no. T5168, Sigma. Secondary: anti-mouse IgG (A9044; Sigma-Aldrich); and goat anti-rabbit IgG peroxidase conjugate (A0545; Sigma-Aldrich).
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2

Molecular Mechanisms Regulating Ca2+ Homeostasis

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HMGB1, PP2, CGP77675, SKF96365, 2-APB, thapsigargin (TG) and dimethyl sulfoxide (DMSO) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Fluo-4/AM and CCK-8 Kits were purchased from Dojindo Laboratories (Kumamoto, Japan). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were from Gibco (Grand Island, NY, USA). Lipofectamine 2000 was from Invitrogen (Carlsbad, CA, USA). FITC-labeled secondary antibodies, mouse monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody and 4',6-diamidino-2-phenylindole (DAPI) were from Santa Cruz Biotechnology (CA, USA). Rabbit polyclonal anti-VE-cadherin antibody, rabbit polyclonal anti-Na,K-ATPase α1 antibody, horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibody, rabbit polyclonal anti-phospho-Src antibody and rabbit polyclonal anti-Src antibody were from Cell Signaling Technology (Beverly, MA, USA). Rabbit polyclonal anti-STIM1 antibody, and anti-human Orai1 antibody were from Abcam (Cambridge, MA, USA).
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