Gb11285

Manufactured by Wuhan Servicebio Technology

Sourced in China

The GB11285 is a laboratory equipment designed for various scientific applications. It serves as a reliable and versatile tool for researchers and scientists. The core function of this product is to perform specific tasks within the laboratory environment. However, a detailed description while maintaining an unbiased and factual approach is not available at this time.

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Lab products found in correlation

5 protocols using gb11285

Evaluating ECM, Inflammatory Markers, and Macrophages

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To evaluate in vivo changes in ECM secretion, levels of
inflammatory cytokines, and macrophage polarization, the sections were
immunohistochemically stained. Briefly, deparaffinized sections were
incubated with 3% H₂O₂ for 15 min in the dark and then
blocked with 3% BSA for 30 min at room temperature. The sections were
incubated overnight at 4°C along with the following primary antibodies: Col
II (1:500, GB111629, Servicebio), aggrecan (1:500, GB11373, Servicebio),
IL-1β (1:200, AF5103, Affinity), IL-6 (1:200, DF6087, Affinity), IL-10
(1:200, DF6894, Affinity), IL-4 (1:200, AF5142, Affinity), CD68 (1:300,
GB113109, Servicebio), Arg1 (1:500, GB11285, Servicebio), and CC-chemokine
receptor 7 (CCR7) (1:300, GB11502, Servicebio). After primary antibody
incubation, the sections were incubated with a specific HRP-conjugated
secondary antibody (1:200, S0001, Affinity) for 1 h at 25°C. The sections
were stained with 3,3′-diaminobenzidine and counterstained with hematoxylin
for the nucleus. Finally, the sections were mounted and photographed using
an optical microscope (Nikon, Tokyo, Japan). Immunohistochemical staining
intensity was digitally quantified using ImageJ software.

Li W., Zhou P., Yan B., Qi M., Chen Y., Shang L., Guan J., Zhang L, & Mao Y. (2023). Disc regeneration by injectable fucoidan-methacrylated dextran hydrogels through mechanical transduction and macrophage immunomodulation. Journal of Tissue Engineering, 14, 20417314231180050.

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Immunofluorescent Staining of Lung Tissue

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The lung tissue was fixed in 4% paraformaldehyde for 1 hour at 4°C, blocked in 20% goat serum for 2 hours at 4°C, and incubated overnight with the antibodies against GATM (GB113430, Servicebio), CD68 (GB113109, Servicebio), and Arginase 1 (GB11285, Servicebio) at 4°C, and then with fluorescein isothiocyanate-labeled secondary antibody (GB22303, Servicebio), at room temperature for 1 hour. We then used a fluorescent triple staining kit (G1236-100T, Servicebio). In addition, DAPI (G1012, Servicebio) was used to stain the nuclei and anti-fluorescence quenching sealer (G1401, Servicebio). The staining was observed under an inverted fluorescence microscope (Servicebio).

Yu L., Wang L., Hu G., Ren L., Qiu C., Li S., Zhou X., Chen S, & Chen R. (2022). Reprogramming alternative macrophage polarization by GATM-mediated endogenous creatine synthesis: A potential target for HDM-induced asthma treatment. Frontiers in Immunology, 13, 937331.

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Bone Tissue Histological Analysis

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The mandible samples were decalcified in a fast decalcified solution (Biotech, China) for 24 h at room temperature, dehydrated in graded ethanol and then embedded in wax. The tissue was cut into 5 μm sections using a microtome (Leica) and stained with HE, Masson’s trichrome and TRAP with commercial staining kits (Servicebio) to assess new bone formation and osteoclast function. After being blocked with bovine serum albumin (Servicebio), the sections were incubated with primary antibodies against Arg-1 (1:500, GB11285, Servicebio) and Runx2 (1:500, GB11264, Servicebio) as well as CD86 (1:500, 26903-1-AP, Proteintech) and Osx (1:500, ab209484, Abcam) at 4 °C overnight. Then, the sections were incubated with appropriate secondary antibodies (Servicebio) before mounting with DAPI. Samples were imaged using a DMI8 inverted fluorescence microscope (Leica Microsystems, Germany) and an LSM980 laser scanning confocal microscope with quantitative analysis by ImageJ.

Yang J., Shuai J., Siow L., Lu J., Sun M., An W., Yu M., Wang B, & Chen Q. (2024). MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment. Bone Research, 12, 2.

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Spinal Cord Injury Histology

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Euthanasia of rats by 2% sodium pentobarbital injection followed by intracardiac perfusion fixation with PBS and 4% paraformaldehyde. The spinal cord was embedded in paraffin and then cut into 20 µm thick sections using electric slicer (Leica, Germany). HE and immunofluorescence staining using primary antibody including anti‐iNOS antibody (GB11119, Servicebio, China), anti‐F4/80 antibody (GB113373, Servicebio, China), anti‐ARG1 antibody (GB11285, Servicebio, China), anti‐CD31 antibody (GB11063, Servicebio, China), anti‐Tuj‐1 antibody (GB12139, Servicebio, China), anti‐GFAP antibody (GB11096, Servicebio, China), anti‐GAP43 antibody (GB11095, Servicebio, China), anti‐NG2 antibody (GB115534, Servicebio, China), anti‐TOMM20 antibody (GB111481, Servicebio, China), anti‐p‐P70S6K antibody (AP0564, Abclonal, China), anti‐NeuN antibody (Servicebio, China) in samples were used to evaluate lesion cavity and nerve regeneration.

Jiang X., Wang W., Tang J., Han M., Xu Y., Zhang L., Wu J., Huang Y., Ding Z., Sun H., Xi K., Gu Y, & Chen L. (2023). Ligand‐Screened Cerium‐Based MOF Microcapsules Promote Nerve Regeneration via Mitochondrial Energy Supply. Advanced Science, 11(6), 2306780.

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Protein Expression Analysis in BMDMs

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Total and nuclear proteins were extracted from BMDMs in RIPA buffer containing protease inhibitors (Servicebio, Wuhan, China). Then protein extracts were separated by 10% SDS-PAGE and incubated overnight at 4°C with primary antibodies specific for AhR (BA2013, 96 kDa, 1:200, Boster, China), HIF-1α (PB9253, 97 kDa, 1:1,000, Boster, China), IRF1 (abs118047, 37 kDa, 1:1,000, Absin, China), NF-κB p65 (GB11142-1, 65 kDa, 1:1,000, Servicebio, China), iNOS (BA0362, 130 kDa, 1:200, Boster, China), Arg-1 (GB11285, 40 kDa, 1:5,000, Servicebio, China), H3 (GB13488, 15 kDa, 1:1,000, Servicebio, China), and β-actin (BA2305, 43 kDa, 1:1,000, Boster, China). Blottings were then probed with appropriate secondary antibodies for 2 h at 25°C and assessed via an ECL kit (Millipore, MA, USA). Triplicate analyses of all samples were conducted.

Yang X., Liu H., Ye T., Duan C., Lv P., Wu X., Liu J., Jiang K., Lu H., Yang H., Xia D., Peng E., Chen Z., Tang K, & Ye Z. (2020). AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization. Theranostics, 10(26), 12011-12025.

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