Tu e cryotitan

Manufactured by Thermo Fisher Scientific

Sourced in United States

The TU/e CryoTitan is a lab equipment product designed for cryogenic applications. It functions as a high-performance cryogenic cooling system.

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Lab products found in correlation

Tcs sp8 x, by Leica (2 mentions) Vitrobot mark 3, by Thermo Fisher Scientific (1 mentions) Spark 10m microplate reader, by Tecan (1 mentions) Nano zsp, by Malvern Panalytical (1 mentions) Fei quanta 200 3d feg, by Thermo Fisher Scientific (1 mentions) Vitrobot mark 4, by Thermo Fisher Scientific (1 mentions) Safire2, by Tecan (1 mentions) Quanta 200 3d feg, by Thermo Fisher Scientific (1 mentions) Prominence 1 gpc system, by Shimadzu (1 mentions)

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CryoTITAN (8 citations)

6 protocols using tu e cryotitan

CryoTEM Imaging of Graphene Oxide Samples

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Transmission electron microscopy at cryogenic
temperatures (CryoTEM) was done using the TU/e CryoTitan (FEI Company).^{38 (link)} Graphene oxide grids, prepared by adding a single
layer graphene sheet to a Quantifoil grid,^{39 (link)} were used for sampling via an automated robot (Vitrobot Mark III,
FEI Company), which was kept at room temperature. The excess of liquid
sample on the grid was blotted away with filter paper to form a thin
film of the dispersion. The thin layer of liquid on the grid was plunged
rapidly into liquid ethane. The vitrification was done in 100% humidity
atmosphere. A tilt series of 27 cryoTEM images from −65°
to +65° with 5° steps were taken to reconstruct the 3D structure
of the particles using IMOD via patch tracking alignment and AVIZO
9.0 software.^{40 (link)}

Najafi M., Kordalivand N., Moradi M.A., van den Dikkenberg J., Fokkink R., Friedrich H., Sommerdijk N.A., Hembury M, & Vermonden T. (2018). Native Chemical Ligation for Cross-Linking of Flower-Like Micelles. Biomacromolecules, 19(9), 3766-3775.

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Cryo-TEM Dose Exposure Protocol

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The samples were loaded into the TU/e
Cryo Titan (FEI Company; now Thermo Fisher Scientific), which was
operated at 300 kV and is equipped with a field emission gun. Diffraction
patterns were acquired at dose rates of 0.1, 1, 10 e/(Å² s), with exposure times of 5, 0.5, and 0.05 s (keeping the total
dose per pattern constant), at a camera length of 1.15 m. For the
bright field imaging experiments, the dose rates were set at 1, 10,
and 100 e/(Å² s), with exposure times of 5, 0.5, and
0.05 s (again, keeping the total dose per image constant), respectively,
at a magnification of 18K. For high accumulated dose experiments,
the screen was flipped down to realize constant irradiation and flipped
up to acquire intermediate images.

Leijten Z.J., Keizer A.D., de With G, & Friedrich H. (2017). Quantitative Analysis of Electron Beam Damage in Organic Thin Films. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 121(19), 10552-10561.

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Cryogenic Electron Microscopy Sample Preparation

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Visualization was conducted using the TU/e CryoTitan (FEI Company). Grids were prepared using an automated robot (Vitrobott Mark III, FEI Company), which was kept at appropriate temperatures between 5 and 35 1C. 20 nm gold nanoparticles were added to an aliquot of the dispersion taken for cryoTEM analysis and are used as fiducial markers for tomography. The excess of liquid on the grid was blotted away with filter paper to form a thin film of the dispersion; and to vitrify, the grid was plunged rapidly into liquid ethane. It was not possible to saturate the vitrobot with a THF-containing atmosphere due to the sensitivity of internal components to THF and the feasibility of maintaining the necessary THF : water ratio within the humidifier. Consequently all samples were prepared in a 100% water atmosphere.

McKenzie B.E., de Visser J.F., Portale G., Hermida-Merino D., Friedrich H., Bomans P.H., Bras W., Monaghan O.R., Holder S.J, & Sommerdijk N.A. (2016). The evolution of bicontinuous polymeric nanospheres in aqueous solution. Soft matter, 12(18).

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Synthesis and Characterization of Amphiphilic AIE Polymersomes

Cited 1 time

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Amphiphilic block-co-polymers PEG-P(AIE) were synthesized according to the previously reported methodology [22 (link)]. To prepare the AIE polymersomes, PEG₄₄-P(AIE)₁₄ was dissolved in THF (2 mg/mL) in a 4 mL glass vial with a magnetic stirring bar and sealed with a rubber septum. After stirring for 10 min, 0.5 mL of ultrapure Milli-Q water was added to the polymer solution through a syringe pump (Chemyx, Inc., Fusion 100, KR Analytical Limited, Stafford, TX, USA) with a speed of 0.25 mL/h. Then, the resulting cloudy solution was transferred into a pre-hydrated dialysis bag (Spectra/Pro^®, MWCO 12,000–14,000, 2 mL/cm, Rancho Dominguez, CA, USA) for dialysis against 0 mM NaCl or 100 mM NaCl solution at 4 °C for at least 24 h. Dynamic light scattering (DLS, Nano ZSP, Malvern Instruments, Malvern, UK), scanning electron microscopy (SEM, FEI Quanta 200 3D FEG, Thermo Fisher Scientific, Waltham, MA, USA), and cryogenic transmission electron microscopy (cryo-TEM, TU/e CryoTitan, Thermo Fisher Scientific, Waltham, MA, USA) were used for size and morphological characterization. For characterization of the fluorescence, a Spark^® 10M microplate reader (TECAN, Männedorf, Switzerland), and two-photon/confocal laser scanning microscopy (TP-CLSM, Leica TCS SP8X, Wetzlar, Germany) were applied.

Shao J., Cao S., Wu H., Abdelmohsen L.K, & van Hest J.C. (2021). Therapeutic Stomatocytes with Aggregation Induced Emission for Intracellular Delivery. Pharmaceutics, 13(11), 1833.

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Cryo-TEM Imaging of Polymersomes

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Experiments were performed on the TU/e cryoTITAN (Thermo Fisher Scientific)
operated at 300 kV equipped with a field emission gun. Grids with
R 2/2 holey carbon film (Cu 200-mesh grids, Quantifoil Micro Tools
GmbH, part of the SPT Life Sciences group) for cryo-TEM measurements
were first plasma treated in a Cressington 208 carbon coater for 40
s before being used. Then, 3 μL of the polymersome solution
was pipetted on the grid and blotted in a Vitrobot MARK IV (Thermo
Fisher Scientific) at 100% humidity. The grid was blotted for 3.5
s (offset −3) and directly plunged and vitrified in liquid
ethane. Processing of TEM images was performed with ImageJ, a program
developed by NIH and available as public domain software at http://rsbweb.nih.gov/ij/.
The nanotube aspect ratio was calculated by dividing the measured
length by the width of each tube and calculating the mean value.

Wauters A.C., Scheerstra J.F., Vermeijlen I.G., Hammink R., Schluck M., Woythe L., Wu H., Albertazzi L., Figdor C.G., Tel J., Abdelmohsen L.K, & van Hest J.C. (2022). Artificial Antigen-Presenting Cell Topology Dictates T Cell Activation. ACS Nano, 16(9), 15072-15085.

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Comprehensive Nanoparticle Characterization Protocol

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The copolymers were analyzed with a Bruker AV 400 MHz Ultra-shieldTM spectrometer and Prominence-I GPC system (Shimadzu) with a PL gel 5 μm mixed D (Polymer Laboratories), equipped with a RID-20A differential refractive index detector. The hydrodynamic size and dispersity index (PDI) of the nanoparticles were determined by a Malvern instruments Zetasizer (model Nano ZSP) dynamic light scattering (DLS) equipped with a 633 nm He-Ne laser and avalanche photodiode detector. The morphologies of the formed nanoparticles were recorded with a FEI Quanta 200 3D FEG scanning electron microscopy (SEM). Cryogenic transmission electron microscopy (cryo-TEM) and cryo-electron tomography (cryo-ET) experiments were conducted on the TU/e CryoTITAN (Thermo Fisher Scientific) equipped with a fieldemission gun operating at 300 kV, an autoloader station and a post-column Gatan energy filter. Fluorescent images were recorded using a Confocal Laser Scanning Microscopy (CLSM, Leica TCS SP8X) equipped with two-photon laser source (Chameleon Vision, Coherent, USA). Cell viability was evaluated via a microplate reader (Safire2, TECAN). Nanosight Tracking Analysis was performed on a Nanosight NS300 equipped with a laser channel (488 nm) and sCMOS camera.

Wang J., Wu H., Zhu X., Zwolsman R., Hofstraat S.R.J., Li Y., Luo Y., Joosten R.R.M., Friedrich H., Cao S., Abdelmohsen L.K.E.A., Shao J, & van Hest J.C.M. (2024). Ultrafast light-activated polymeric nanomotors. Nature communications, 15(1).

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