MTX, 7-OH MTX, and d3-MTX were purchased from Dalian Meilun Biotech Co. (Dalian, China). NIM, phthalazine, and protease inhibitor cocktail were obtained from Sigma-Aldrich (St. Louis, MO, USA). The 4′-hydroxynimesulide (M1), nitro-reduced nimesulide (M2), and acetylated metabolite of nitro-reduced nimesulide (M4) were synthesized as previously described [19 (link)]. SGX523 and JNJ-38877606 were purchased from Selleck Chemicals (Houston, TX, USA). All reagents used in the cell culture were supplied by Invitrogen (Carlsbad, CA, USA). A bicinchoninic acid assay (BCA) protein assay kit and radioimmunoprecipitation assay (RIPA) buffer were purchased from Beyotime (Shanghai, China). The rabbit anti-AOX1 polyclonal antibody and goat anti-AOX3 polyclonal antibody were obtained from Santa Cruz (Dallas, CA, USA). The rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) monoclonal antibody was purchased from CST (Danvers, MA, USA). All other reagents and solvents were either of analytical or high-performance liquid chromatography grade.
Phthalazine
Manufactured by Merck Group
Sourced in United States
Phthalazine is a heterocyclic organic compound used as a laboratory reagent. It is a colorless, crystalline solid that is soluble in various organic solvents. Phthalazine serves as a building block for the synthesis of other chemical compounds and is utilized in various analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Sgx 523, by Selleck Chemicals (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Bicinchoninic acid assay bca protein assay kit, by Beyotime (1 mentions)
Radio immunoprecipitation assay ripa buffer, by Beyotime (1 mentions)
Pyridazine, by Merck Group (1 mentions)
Unilab glovebox, by MBraun (1 mentions)
Methanol d4, by Merck Group (1 mentions)
Phenacetin, by Merck Group (1 mentions)
Chlorzoxazone, by Merck Group (1 mentions)
Superoxide dismutase, by Merck Group (1 mentions)
Catalase, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Avantor (1 mentions)
Potassium phosphate monobasic, by Avantor (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
3 protocols using phthalazine
1
Quantification of Methotrexate and Metabolites
2
Schlenk Synthesis of Iridium Complex 1
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All the experimental procedures associated with this work were carried out under nitrogen using standard Schlenk techniques or in an MBraun Unilab glovebox. The solvents used in the synthetic chemistry were dried using an Innovative Technology anhydrous solvent system, or distilled from an appropriate drying agent under nitrogen. Methanol-d4, pyridazine and phthalazine were obtained from Sigma-Aldrich and used as supplied. [Ir(IMes)(COD)Cl] (1 ) was prepared according to literature methods.45
3
In Vitro Metabolism of Benzylguanine and Zaleplon
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O 6 -benzylguanine (O6BG), its metabolite, 8-oxo-benzylguanine, and zaleplon were purchased from Toronto Research Chemicals (North York, ON). N-(2-dimethylamino)ethyl)acridine-4-carboxamide (DACA) and its metabolite (DACA-acridone) were synthesized as described previously 23 . Phenacetin as the internal standard, catalase, phthalazine, 1-phthalazinone, chlorzoxazone (CZN), and 3,4-Methylenedioxymethamphetamine (MDMA) were purchased from Sigma-Aldrich (St. Louis, MO). 5-oxozaleplon was biosynthesized and puri ed as previously described 23 . Dihydroxymethamphetamine (DHMA) was purchased from Cayman Chemical (Ann Arbor, MI). CYP2D6 BACULOSOMES® Plus Reagents and tris(2-carboxyethyl) phosphine were purchased from Thermo Fisher (Waltham, MA). Formic acid was purchased from Fischer Scienti c (Pittsburgh, PA). Acetic acid, acetonitrile, superoxide dismutase, and NADPH were purchased from EMD Millipore Corp. (Billerica, MA) and EMD Chemicals (San Diego, CA), respectively. The monobasic potassium phosphate, dibasic potassium phosphate, and EDTA used for the potassium phosphate buffer were purchased from JT Baker (Center Valley, PA).
Human CYP 2E1 Supersomes TM was purchased from BD Gentest (Franklin Lakes, NJ). Expression and puri cation of recombinant human aldehyde oxidase (hAO) was performed following the method previously described 23, 24 .
Human CYP 2E1 Supersomes TM was purchased from BD Gentest (Franklin Lakes, NJ). Expression and puri cation of recombinant human aldehyde oxidase (hAO) was performed following the method previously described 23, 24 .
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