Hdl c

The levels of alanine transaminase (ALT) (cat. #140120006, Mindray), aspartate aminotransferase (AST) (cat. #140220005, Mindray), Alkaline Phosphatase (ALP) (cat. #140320004, Mindray), total cholesterol (TC) (cat. #141620006, Mindray), triglyceride (TG) (cat. #140120006, Mindray), high-density lipoprotein cholesterol (HDL-C) (cat. #142120005, Mindray), and low-density lipoprotein cholesterol (LDL-C) (cat. #142020004, Mindray) in the serum were measured using standard analytics.

Automated Haematology analyzer (Mindray, China), Chemistry Analyzer (Mindray, China), total cholesterol (TChol), triglyceride (TRG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), bilirubin (total and direct), urea, creatinine, sodium, potassium, chloride reagents, diluent, and lyse all from Shenzhen Mindray Bio-Medical Electronics Co. Ltd., China, 80-2C centrifuge, urine strips (Urit Medical Electronics Co. Ltd., China), ethylene diamine tetra acetic acid (EDTA) tubes, and serum gel separator tubes (Greenlife, Canada), Sprague–Dawley rats were purchased from Centre for Plant Medicine Research (CPMR), Mampong Akwapim, Ghana. All reagents were analytical grade and solutions freshly prepared.

The lipid profile tests measure serum total cholesterol (TC), triglyceride (TG), HDL-C, LDL-C, and VLDL. After a 12–14 h overnight fasting, 5 ml of blood sample was collected under aseptic precautions in Serum Separator Tubes. To prepare the serum, the collected blood samples were clotted for 1 h and then centrifuged for 10 min, 3000 rpm at room temperature. The serum samples were stored in the freezer −80 °C until measurements. The serums were then analyzed for lipid profile tests by the commercially available kits, Cholesterol (Pars Azmoon, Iran), Triglyceride (biorexfars, Iran) and HDL-C (PishtazTeb, Iran) using a biochemistry autoanalyzer (BS-200 E, mindray, USA). The levels of LDL-C and VLDL were calculated by the following formula: