Andrographolide

Manufactured by Solarbio

Sourced in China

Andrographolide is a bioactive compound derived from the Andrographis paniculata plant. It is a major constituent of the plant and has been widely studied for its potential medicinal properties. Andrographolide is commonly used in various research applications, particularly in the field of natural product chemistry and pharmaceutical development. As a laboratory reagent, it can be utilized for analytical and experimental purposes.

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Lab products found in correlation

2 protocols using andrographolide

Cell Culture and Compound Preparation Protocol

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Dulbecco’s
modified Eagle’s
medium (DMEM), trypsin with/without ethylenediaminetetraacetic acid
(EDTA), penicillin–streptomycin, and sterile phosphate-buffered
saline (PBS) were obtained from Solarbio Co., Ltd. (Beijing, China).
Reduced GSH, quercetin, rutin, andrographolide, resveratrol, polydatin,
curcumin, and ursolic acid were obtained from Solarbio Co., Ltd. (Beijing,
China), and all of the plant components are standards with purity
greater than 99%. Fetal bovine serum (FBS) was purchased from Gibico
(Gaithersburg, MD). quercetin stock (25 mM) was dissolved in dimethyl
sulfoxide (DMSO) and preserved under −20 °C. Dextran sulfate
sodium salt (DSS, 36 000–50 000 Da) was purchased
from MP Biomedicals.

Dong Y., Hou Q., Lei J., Wolf P.G., Ayansola H, & Zhang B. (2020). Quercetin Alleviates Intestinal Oxidative Damage Induced by H2O2 via Modulation of GSH: In Vitro Screening and In Vivo Evaluation in a Colitis Model of Mice. ACS Omega, 5(14), 8334-8346.

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Andrographolide Cytotoxicity Evaluation

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Andrographolide (Solarbio, Beijing, China) with a molecular weight of 350.45 was diluted to 100 mM with DMSO and used as the stock solution. Serum-free Dulbecco's Modified Eagle's Medium (DMEM) (Gibco, USA) was used to dilute the APL master mix. Thiazolyl blue tetrazolium bromide (MTT) was sourced from Solarbio in Beijing, China, and Dimethyl sulfoxide (DMSO) was obtained from Sigma-Aldrich in Shanghai, China. Additionally, DMEM 2X High Glucose with L- glutamine and sodium pyruvate was procured from Gibco in the USA, Methylcellulose (MC) was provided by Solarbio in Beijing, China, and a 1% Crystal Violet Staining Solution was also obtained from Solarbio in Beijing, China.

Shen J., Xu Q., Chen L., Chang X., Shen R., Zhao Z., Zhu L., Wu Y, & Hou X. (2023). Andrographolide inhibits infectious bronchitis virus-induced apoptosis, pyroptosis, and inflammation. Antiviral therapy, 28(5).

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